What is NOT a feature of PCR? O a. The target can be much larger than the primers. Ob. Primers do not have to be perfectly matched to flanking sequences to amplify targets. O PCR is highly specific because of the stringency of hybridization at relatively high temperature. Od. The sequence of the target needs to be known so that complementary primers can be synthesized. Oe. PCR is exquisitely sensitive; a single DNA molecule can be amplified and detected.

General, Organic, and Biological Chemistry
7th Edition
ISBN:9781285853918
Author:H. Stephen Stoker
Publisher:H. Stephen Stoker
Chapter22: Nucleic Acids
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Problem 22.84EP
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What is NOT a feature of PCR?
O a. The target can be much larger than the primers.
Ob. Primers do not have to be perfectly matched to flanking sequences to amplify targets.
O. PCR is highly specific because of the stringency of hybridization at relatively high temperature.
d. The sequence of the target needs to be known so that complementary primers can be synthesized.
Oe. PCR is exquisitely sensitive; a single DNA molecule can be amplified and detected.
Transcribed Image Text:What is NOT a feature of PCR? O a. The target can be much larger than the primers. Ob. Primers do not have to be perfectly matched to flanking sequences to amplify targets. O. PCR is highly specific because of the stringency of hybridization at relatively high temperature. d. The sequence of the target needs to be known so that complementary primers can be synthesized. Oe. PCR is exquisitely sensitive; a single DNA molecule can be amplified and detected.
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