What is a recombinant DNA vaccine? List two such vaccines. State their advantages.

d,e,f

Our Western Blot experiment requires a buffer called TBS. At working concentration (1X), this buffer contains: 20 mM Tris 150 mM NaC1. Prior to use, however, the buffer is made and stored as a 10X concentrated stock What are the concentrations of these two ingredients in the 10X stock? Primary antibodies for the Western Blot experiment are prepared using this TBS buffer, with the addition of several other ingredients. Calculate the amount of each ingredient needed to produce10 ml of the final working antibody solution. b. 10X TBS stock c 0.01% (v/v) Tween detergent d. 3% (w/v) BSA (molecular weight 66430 g/mol) e. 1:10,000 dilution of antibody stock

Compared to an RNA Vaccine what additional steps does the information in DNA vaccine need to undergo before an antigenic protien is produced?