Biochemistry
Biochemistry
9th Edition
ISBN: 9781319114671
Author: Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher: W. H. Freeman
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Question

1. What chemical is causing the initial absorbance in the spectrophotometer (enzyme assay for determination of glucose and fructose concentrations), before any enzymes are added? Pick the best choice.    NADP+,  Beverage sample, ADP, NADPH, Imidazole buffer, Fructose, G6P dehydrogenase, Water, Hexokinase, Glucose, ATP, Phosphoglucoisomerase

2. After the enzymes have been added and the reaction has occurred, what chemical is being measured in the spectrophotometer (enzyme assay for determination of glucose and fructose concentrations)? Pick the best choice.   NADP+,  Beverage sample, ADP, NADPH, Imidazole buffer, Fructose, G6P dehydrogenase, Water, Hexokinase, Glucose, ATP, Phosphoglucoisomerase

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Step 1: Enzyme assay for determination of glucose and fructose concentration
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Step 2: Spectrophotometric analysis during the enzyme assay
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Step 3: 1. Chemical causing background radiation
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Step 4: 2. The analyte whose absorbance is measured
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