What are the two type of viral vector involved in gene therapy trials?

Q: name  the six different types of vectors?

A: Vector is DNA molecule that is used as a vehicle to artificially carry the gene of interest using…

Q: Describe the methods that are being used to introducegenes into humans for gene therapy.

A: Gene therapy is a clinical field which centers around the usage of the remedial conveyance of…

Q: How do you clone a 4 kb promoter from a genomic cell preparation?

A: Genome is the complete set of genetic material present in the nucleus of a cell. It houses all the…

Q: When researchers cloned the ey, Pax-6, and AN genes, What did they found?

A: Introduction Pax-6 stands for Paired Box Protein-6. Pax is the group of gene that lead a main role…

Q: What is the definition of antibiotic resistance gene?

A: Antibiotics are the antimicrobial chemicals that can be bactericidal (kill the bacteria) or…

Q: What is the purpose of the targeting vector?

A: In molecular cloning, vector is a segment of DNA molecule, which is used as an artificial carrier of…

Q: How might genomic information be helpful for the effective use of imatinib mesylate (Gleevec) in…

A: Imatinib mesylate is a drug used for the treatment of myeloid leukemia that is caused due to…

Q: What are the application of rice genome?

A: Introduction  Domestic Rice (Oryza sativa) is the most important cereal crops. It is the staple food…

Q: what are the Resistant strain emergence to DNA inhibitors

A: Drug resistance is the reduction in effectiveness of a medication such as antimicrobial in treating…

Q: Describe the method of pronuclear injection to generate transgenic mice?

A: Transgenic mice are produced by putting DNA in their genome from another source. There are a number…

Q: What are the advantages of normalizing against a reference gene in qPCR?

A: PCR stands for Polymerase Chain Reaction, and it is a widely used method that allows us to make many…

Q: How is a gene inserted into a plasmid cloning vector?

A: Apart from cell's chromosomal DNA, a double-stranded circular DNA molecule is present in yeast,…

Q: Discuss how to transform the yeast vector, YEP containing your gene of interest into the host cell.

A: To insert a set of desirable foreign genes into the host genome for replication and expression, a…

Q: Will genome editing emerge as the safest and most reliablemethod of gene therapy, rendering other…

A: Genome editing is an advanced molecular technique where the DNA sequences are altered through the…

Q: Describe procedure of RFLP.

A: RFLP (restriction fragment length polymorphism) is an inherited difference in the pattern of…

Q: How was it experimentally proven that the spacer sequences in the CRISPR locus were the elements…

A: CRISPR-cas system provides an adaptive defence mechanism to the prokaryotes which acquires…

Q: Discuss the four important features of DNA vectors.

A: Vector usually is a small DNA molecule which is stable and it can be easily isolated.  A vector…

Q: plain the fundamental difference between CRISPR/Cas9 and in vivo adenovirus-based gene therapy

A: All biological data of AN organism is held on within the biomolecule known as DNA. gene is the…

Q: What is a mouse model for human disease?

A: Humans are the multicellular eukaryotic organism belonging to the kingdom Animalia and class…

Q: What is o A vector derived from the tumor-inducing (Ti) plasmid of the bacterium Agrobacterium…

A: In recombinant technology, vectors are the DNA molecules that are used as a vehicle which carry…

Q: Discuss about Genome 10K Project ?

A: The advent of low cost, high throughput sequencing has ushered in a new age of genome science and…

Q: Describe how a plaque assay is used to detect and estimate the numbers of phage particles.

A: Plaque is a term used in microbiology for a clear zone on a bacterial culture plate that indicates…

Q: What is commonly used as a vector for introducing a DNA fragment in human lymphocytes?

A: Gene therapy is a method that allows the correction of the defective gene. In gene therapy, it is…

Q: Does CRISPR-Cas9 can be replaced p1vir transduction method to create the mutations？why？

A: CRISPR-Cas9 is a powerful genome editing tool that enables scientists to make precise changes to the…

Q: What are knockout mice, how are they produced, and for what are they used?

A: The gene knockout is a mechanism in which one of the genes of an organism is made inoperative. The…

Q: How can we use the transposon sequence to find where the mutation occurred in a forward genetic…

A: Forward genetic screes, also termed as forwarding genetics, is a kind of process by which those…

Q: Define how DNA can be inserted into vectors ?

A:

Q: Describe a method of making transgenic plants.

A: A transgenic plant has a gene sequence that is naturally not found in its genome.

Q: Explain the several key properties of DNA vectors ?

A: Microbiology is the scientific study of single celled, multicellular, and acellular microorganisms.…

Q: What is the difference between gene therapy and CRISPR?

A: The process by which scientists can manipulate the genetic material of the organism in the…

Q: How can gene therapy do to help in the cure of COVID-19?

A: Gene therapy is an experimental medical technique in which DNA (deoxyribonucleic acid) is introduced…

Q: What is the motive behind Genome Project 10K?

A: The advent of low-cost, high throughput sequencing has ushered in a new age of genome science and…

Q: What kind of DNA is actually used to make the spots for analysis for the Vicrochip DNA microassay?

A: DNA (deoxyribonucleic acid) microarray or DNA chip is a technique that is used to analyze the…

Q: Explain how you would compare the expression of a gene in a cancer cell and a normal cell using…

A: The functions of a gene can be studied by looking at how it expresses in a specific cell and how it…

Q: Name several types of vectors, and list the types of genes they needto function.

A: In molecular cloning, the vector is a Deoxyribose Nucleic Acid (DNA) particle that fills in as the…

Q: If want to express the gene from monkey, the PET vector or BACMAN would be best? Briefly explain

A: The decision between PET and BACMAN vectors for expressing a gene from a monkey depends on several…

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