Which of the following is TRUE about immunofluorescence microscopy where we localize proteins using fluorescently tagged antibodies? Pick the best answer: We can use it to resolve 2 points that are 10 nanometers apart. The emission wavelength of the fluorophore is typically shorter than the excitation wavelength. The resolution can be further improved with increased magnification. All else being equal, a fluorophore that emits red light will give better resolution than a fluorophore that emits green light. Based on what we discussed in class, we can use the technique on live samples therefore enabling us to see the dynamic movement of cytosolic proteins in living cells. None of the above

Contrast the overall cell wall compositions of Gram positive and Gram negative bacteria, and go step-by-step in the procedure of the Gram stain. Describe what is occurring during each step within both Gram-positive cells and in Gram-negative cells. Be sure to include the appearance of each cell type at the end of each step.

Isolate #2: Cell Morphology (shape) . a. b. Isolate #2: Gram stain result Gram Postive. (The color I see on my screen is purple.) c. List at least two genera (not species) of bacteria that are consistent with the Gram reaction and morphology you observed. d. Are any of these genera are normal flora on the skin? If so which one(s)? (1 pt) ***Please answer the four questions base on the picture provided.***

What is the primary stain for the Ziehl-Neelson acid fast stain? What color is this stain? What cells will display this stain once you finish the stain and view your slide? Paragraph BIU