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Q: What are STRs, and why are they useful for DNA profiling?
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A: DNA is a genetic material present in every cell.
Q: What is the mom method of pacaking DNA?
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Q: How does PFGE separate larger fragments more efficiently than standard electrophoresis? 2. Why is…
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Q: Explain The replica plating technique?
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Q: What is the useful of the Development of highly sensitive and low-cost DNA agarose gel…
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Q: Why is the annealing temperature different between samples during DNA extraction & marker gene…
A: Introduction Polymerase chain reaction or PCR laboratort technique by which millions to billions of…
Q: What are the key differences between DNA microarrays and protein microarrays, and how they are used…
A: Introduction: A laboratory method used to recognize the expression of thousands of genes…
Q: Who proposed shotgun sequencing?
A: The method used for the sequencing of random DNA strands is genetics, shotgun sequencing. This is…
Q: What is the program Finch TV used for? Viewing next generation sequencing data Opening sequencing…
A: Ans- C EXPLANATION- FINCH Tv is the tool or application used by the researchers to view, analyze,…
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Q: What special kind of nucleotide is used in the Sanger sequencing procedure?
A: A British biochemist named Fred Sanger and few of his research colleagues developed the process of…
Q: What is the purpose of shotgun sequencing?
A: Deoxyribonucleic acid (DNA) sequencing is the process of determining the nucleic acid sequence based…
Q: What different techniques can be used for transcriptomeanalysis ?
A: The transcriptome is the set of all RNA transcripts including coding and non-coding in an individual…
Q: What is the source of agarose, the powder used to make the gel? What is the…
A: Gel electrophoresis is a technique used by scientists to separate DNA or protein molecules. Gel…
Q: what kinds of techniques are used to sequence large fragments of dna upto 50kb
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Q: Why are the proteins cleaved into small fragments for protein sequencing?
A: Proteins are the end product of the molecular translation that codes the information present in the…
Q: Which vectors can be used to clone a continuous fragment of DNA with the following lengths? a. 4 kb…
A: According to the question, we have to mention the name of the vectors that can be used to clone a…
Q: What is the sequence of the sample DNA submitted for sequencing given the gel electrophoresis…
A: DNA is a hereditary molecule found in cells that transmits genetic information from one generation…
Q: Why is extra primers added to the pcr reaction?
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Q: Is it necessary to cut the isolated genomic DNA of an organism with restriction enzymes for…
A: Restriction enzymes are also known as DNA-cutting enzymes which recognizes one or a few target…
Q: What is linear amplification PCR?
A: Molecular biology techniques are the techniques that utilize the genetic material of an organism…
Q: What are the criteria for “good” primers in a PCR reaction?
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Q: What is restriction fragment length polymorphism and which method do we use to detect it?
A: Restrictions length polymorphism (RFLP) is a kind of polymorphism that outcomes from variety in the…
Q: What is the purpose of adding the dishwashing liquid to the DNA extraction?
A: DNA extraction is a method of separating DNA from cell membranes, proteins, and other cellular…
Q: Is it possible to obtain DNA in cloverleaf configuration? Explain
A: Cloverleaf Model: The cloverleaf model of tRNA is a representation of the molecule's structural…
Q: What is the cause of an extra band in a DNA ladder in agarose gel electrophoresis?
A: The following can lead to the formation of an extra band in the DNA ladder lane - 1. Contamination -…
Q: Why is it necessary to use only the Klenow fragment, rather than intact E. coli Pol I, in DNA…
A: A protein fragment that is obtained when the DNA polymerase I of E.coli is enzymatically cleaved…
Q: what is Inverse PCR ?
A: PCR which stands for polymerization chain reaction is an analytic technique which is used to amplify…
Q: how are okazaki fragments created?
A: An Okazaki fragment refers to DNA fragments that are formed during the DNA replication process.
Q: How much 340bp insert DNA should be added to a ligation in which 50ng of 4.3kb vector will be used?…
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Q: What are the principles of Chelex extraction and solid-phase DNA extraction methods?
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Q: Is the DNA soluble in the aqueous solution or alcohol?
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Q: What is DNA microarray used for?
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Q: How does the shotgun approach differ from the whole genome sequencing?
A: Biotechnology is concerned with exploitation of biological agents or their components for generating…
Q: What is a PCR?
A: PCR is a technique widely used in molecular biology and it was developed by Kary Mullis in 1983.
Q: What are the major considerations in the design of PCR cloning primers?
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Q: What is the purpose of adding table salt (NaCl) to the DNA extraction buffer?
A: The deoxyribonucleic acid (DNA) carries the genetic information and is usually present in the…
Q: What is a Sanger Sequencing ?
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Q: How to identify the type of genomic DNA isolation contamination and how to eliminate this…
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Q: What is the purpose of the PCR?
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Q: a) What are vectors? Describe extensively the roles vectors play in genetic engineering? Write short…
A: Note: Solving answer only for part A as per the guidelines, repost remaining part, please. The…
Q: what are the advantages and disadvantages of Gel electrophoresis in DNA analysis
A: Introduction :- Electrophoresis is a technique used in laboratories to separate DNA, RNA, and…
Q: Given the electrophoresis profile of a Sanger sequencing result, what was the sequence of the…
A: The Sanger sequencing method is also called as chain termination method. In this method, dideoxy…
2) What are the techniques to verify the extracted genome?
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- Microarray hybridization is used mostly in transcript profiling or assaying DNA variation. Although the technology for establishing DNA microarrays was developed only recently, numerous applications have already been developed and their impact on future biomedical research and diagnostic approaches is expected to be profound. Give some examples of the practical use of this technique.Describe the rationale for column-based DNA extraction and purification.Explain the Technical Issues Surrounding DNA Profiling ?