What are the questions in the following that should be asked to the patient described above: “Have you ever had a foot or leg ulcer?” “Does your abdomen hurt?” “Is there any drainage from your foot?” “Do you feel any burning on urination?” “Are you using any creams or powders on your skin?” “Do you examine your skin regularly?” “Are you feeling very anxious?” “Have you noticed if your skin is dry, itchy, scaly, flaky, or rough?” “Do you wear sunscreen when you are in the sun?” “What medications are you taking
Molecular Techniques
Molecular techniques are methods employed in molecular biology, genetics, biochemistry, and biophysics to manipulate and analyze nucleic acids (deoxyribonucleic acid (DNA) and ribonucleic acid (RNA)), protein, and lipids. Techniques in molecular biology are employed to investigate the molecular basis for biological activity. These techniques are used to analyze cellular properties, structures, and chemical reactions, with a focus on how certain molecules regulate cellular reactions and growth.
DNA Fingerprinting and Gel Electrophoresis
The genetic makeup of living organisms is shown by a technique known as DNA fingerprinting. The difference is the satellite region of DNA is shown by this process. Alex Jeffreys has invented the process of DNA fingerprinting in 1985. Any biological samples such as blood, hair, saliva, semen can be used for DNA fingerprinting. DNA fingerprinting is also known as DNA profiling or molecular fingerprinting.
Molecular Markers
A known DNA sequence or gene sequence is present on a chromosome, and it is associated with a specific trait or character. It is mainly used as a genetic marker of the molecular marker. The first genetic map was done in a fruit fly, using genes as the first marker. In two categories, molecular markers are classified, classical marker and a DNA marker. A molecular marker is also known as a genetic marker.
DNA Sequencing
The most important feature of DNA (deoxyribonucleic acid) molecules are nucleotide sequences and the identification of genes and their activities. This the reason why scientists have been working to determine the sequences of pieces of DNA covered under the genomic field. The primary objective of the Human Genome Project was to determine the nucleotide sequence of the entire human nuclear genome. DNA sequencing selectively eliminates the introns leading to only exome sequencing that allows proteins coding.
You are a visiting nurse assigned to check on Mr. Green, a widower who lives by himself in a two-story house. Mr. Green is a 66-year-old man who has been an uncontrolled diabetic for the past 15 years. His wife used to provide nutritious meals and help him test his blood sugar levels, as well as observe his skin for any lesions. Since she passed away of cancer 1 year ago, he has been isolated at home and is having trouble taking care of himself. His physician initiated the visiting nurse referral after he missed two appointments. He is alert and oriented but appears depressed to you. He reports that he had a callous on his left heel that was thick and bothersome, so he tried to break it down with a callous file, but it began bleeding about 3 weeks ago. Mr. Green states, “It is hard for me to really check my skin because my eyesight is so poor.” He states that it is hard for him to walk anywhere because he has mild discomfort walking on the left foot. He also states, “My groin is itchy and red.” You start with some focused questions.
What are the questions in the following that should be asked to the patient described above:
- “Have you ever had a foot or leg ulcer?”
- “Does your abdomen hurt?”
- “Is there any drainage from your foot?”
- “Do you feel any burning on urination?”
- “Are you using any creams or powders on your skin?”
- “Do you examine your skin regularly?”
- “Are you feeling very anxious?”
- “Have you noticed if your skin is dry, itchy, scaly, flaky, or rough?”
- “Do you wear sunscreen when you are in the sun?”
- “What medications are you taking
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