What are plasmids? What is the importance of plasmids for the recombinant DNA technology?

What is the most logical sequence of steps for splicing foreign DNA into a plasmid and inserting the plasmid into bacterium?I) Transform bacteria with recombinant DNA molecule.II) Cut the plasmid DNA using restriction enzymes.III) Extract plasmid DNA from bacterial cells.IV) Hydrogen-bond the plasmid DNA to non-plasmid DNA fragments.V) Use ligase to seal plasmid DNA to non-plasmid DNA     A. III, II, IV, V, I       B. I, II, IV, III, V     C. III, IV, V, I, II   D. II, III, V, IV, I

Why is it important to include a selective marker gene on a plasmid? a) So that you can select cells containing the plasmid after transformation. b) So that you can select where the plasmid inserts into the genome. c) So that you can select the recombinant gene you want to add to the plasmid. d) So that you can select when the recombinant gene on your plasmid is expressed.

Why is it important to include a selective marker gene on a plasmid? a) So that you can select cells containing the plasmid after transformation. b) So that you can select where the plasmid inserts into the genome. Oc) So that you can select the recombinant gene you want to add to the plasmid. d) So that you can select when the recombinant gene on your plasmid is expressed.

From where do we get primers for sequencing DNA? A) they are synthesized by reverse transcriptase B) they are cut out of plasmids using restriction endonucleases C) DNA primase is added to the sequencing reaction and synthesizes the primers D) biotechnology companies synthesize them using organic chemistry

What is a plasmid? What are the minimum 3 components that a recombinant plasmid used for genetic transformation should have?

what’s a plasmid?

What are DNA ligases? Howdo these enzymes participatein the recombinant DNAtechnology?

Which of the following would be a good application of plasmid transformation? 1) to make copies of DNA 2) to isolate a change in a single nucleotide  3) to separate DNA fragments 4) to sequence DNA

How does the universality of the genetic code make the recombinant DNA technology possible?