What are the coefficients for the following reaction when it is properly balanced? nitric acid + sodium hydroxide → sodium nitrate + water O 5, 2, 2, 1 O 2,3, 2, 2 O 1, 1, 1, 1 O 2, 1, 1, 1

The students conducted the assay for LDH activity using the two serum samples. Each cuvette (path length 1 cm) contained 3ml of a suitable assay buffer (including pyruvate as substrate). 20 microlitres of either serum was added to the cuvette and the absorbance values immediately recorded at the optimum wavelength for a period of 5 minutes (absorbance readings taken every 30 seconds).   Protein concentration of serum sample (mg/ml) Change in absorbance at optimum wavelength per minute Control serum (C) 8 -0.04 Diseased serum (D) 7.8 -0.6 1c. Using the molar absorption coefficient of NADH as 6220 M-1 cm-1, and by application of the Beer-Lambert law, estimate the enzyme activity in the two samples (C and D). Express activity as moles per second.  1d. Estimate the specific activity of the two samples (moles per second per microgram).

Sel Absorbance S Verify your molar absorptivity value. Did you report your data to the correct number of significant figures? Analytical wavelength Analytical wavelength for blue dye (nm) Calibration curve Solution 1 2 3 4 5 Dye concentration (μM) 15.20 7.51 Unknown solution 3.74 1.89 1.01 Molar absorptivity (μM ¹cm-¹) Absorbance Concentration of unknown blue dye (µM) 629 Absorbance 0.756 0.432 0.259 0.175 0.144 0.083 How to calculate concentration from absorbance S A d tra 01

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