Tube Tris/CaCl, pH8 (ml) BAPNA Trypsin (µL) (µL) Final [BAPNA] (mM) 1 2.895 5.0 100 2 2.89 10.0 100 3 2.875 25.0 100 4 2.85 50.0 100 5 2.80 100 100 6 2.70 200 100 7 2.50 400 100 8 2.10 800 100
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![Tube
Tris/CaCl, pH8 (ml) BAPNA
Trypsin (µL)
(µL)
Final [BAPNA]
(mM)
1
2.895
5.0
100
2
2.89
10.0
100
3
2.875
25.0
100
4
2.85
50.0
100
5
2.80
100
100
6
2.70
200
100
7
2.50
400
100
8
2.10
800
100](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2Fff63bb05-9df4-4fe5-b1ca-eb1247f8ed64%2F6b188c71-afc1-47f2-810e-b07e0fea8509%2F9r3oyip_processed.jpeg&w=3840&q=75)
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- 1s²2s²2p¹4) Paxlovid is treatment for COVID-19 that was recently approved for use because it can drastically reduce the risk for hospitalization and death when taken early in the infection. It is actually a package of two different antiviral drugs. One of those is Nirmatrelvir, which is a modified tripeptide protein mimetic designed to covalently bind to and inhibit the main protease (MPro) from the SARS-CoV-2 virus. This protease recognizes and hydrolyzes peptide bonds at specific points in their sequence, and it is essential for viral replication. a. Below is one of the sequences that Mpro recognizes and cleaves. It cleaves the peptide bond between the glutamine and the glycine. Draw out the structure of a peptide with this sequence, including the appropriate stereochemistry for biological amino acids and assuming the peptide is at physiological pH (pH = 7.4). SGVTFQGKF b. What would the net charge of the peptide be at physiological pH?. c. At what pH would the peptide be net neutral?Susceptibility to spoilage is affected by water content of the food. Why? please explain very detail
- The image below is a positive ionization mode Electrospray ionization-mass spectrum (ESI-MS) spectrum for a peptide, obtained using high resolution mass spectrometry. If ionization occurs through de-protonation (assuming that the mass of a proton is 1.0 Da), what is the neutral mass of the peptide, in Da? 627.271 627.604 100 - 627.938 628.271 628.604 628.938 627.0 628.0 629.0 mass/charge (m/z) 628.271 626.271 1878.813 O 1884.813 % AbundanceMost of the ultraviolet absorption of proteins at 280 nm is due to their content of a. Glutamate b. Tryptophan c. Alanine d. Aspartated) 1.EtMgBr PCC HO- CH2CI2 2. H20 A
- 23. Enzyme activity is best determined by performing a(n) a. enzyme international unit b. enzyme efficiency test C. enzyme specificity test d. enzyme assayvo(pM/min) 100 90 80 70 60 50 40 30, 20 10 0 What is V max for this enzyme ? Enzyme concentration 0.1 µM 5 10 15 20 A. 100 mm/min B. 1.0 mM/min C. 0.5 μM/min D. 50 µM/min E. 90 µM/min 25 30 35 37Concentration Sof nicotinamide mono- nucleotide, in mM 0.138 0.220 0.291 0.560 0.766 1.460 a. 0.6482 b. 0.5853 0.4129 d. 0.4682 e. 0.9214 Initial rate vof nicotinamide- adenine dinucleotide formed, in micromoles Construct the Lineweaver-Burk linear plot and find the value of v C. 0.148 0.171 0.234 0.324 0.390 0.493 worksheet 16-Go... Microsoft Excel (P TSU
- One of the most advanced methods for determining a protein sequence is tandem mass spectrometry (MS/MS). Select from the list of statements below all that applies to MS/MS. Group of answer choices CID is one way of performing tandem MS experiments The experiment start by chopping the protein into smaller peptide fragments using an enzyme These peptide fragments are later introduced into the mass spectrometer to determine their molecular weight Peptide fragments are selected for further fragmentation by collision with an inert gas There are only 4 types of ions which can result from MS/MS fragmentation: a, b, x, and y ions The a and b ions are the ones with the original C terminus, whereas the x and y ions are the ones with the original N-terminus All of the aboveThe Nat-Ca2+ exchanger is used to help regulate cardiac muscle function. It transports three Na* into the cell and one Ca²+ out of the cell in every cycle. What kind of transporter is the Nat-Ca2+ exchanger? Choose one: OA. Uniport B. Symport O C. Antiport Part 2 Digoxin is a drug used to treat heart failure by blocking the Nat-K* pump in cardiac cells. How would inhibition of the Nat-K+ pump alter the intracellular concentration of Ca²+?The NFPA Hazard Identification System in the label below signifies. (attached photo) a.Extreme Danger, Above 100 ⁰F Not Exceeding 200 ⁰F, Violent Chemical Change, Acid b.Extreme Danger, Above 100 ⁰F Not Exceeding 200 ⁰F, Unstable if Heated, Corrosive c.Extreme Danger, Above 200 ⁰F, Violent Chemical Change, Acid d. Extreme Danger, Above 100 ⁰F Not Exceeding 200 ⁰F, Violent Chemical Change, Corrosive e. none of the above
