What are the basic medium, acid medium, and zwitterion formulas of phenylalanine?

Q: 9. The water-soluble vitamins B₁ (thiamine hydrochloride), B₂ (riboflavin), B3 (niacinamide), and B…

A: According to the given pKa value and the following structure, the order of elution for the four B…

Q: general mechanism of electron ionization

A: 1 Electron ionisation is a part of mass spectroscopy. 2. the key points here are Analyte…

Q: Metal ions chelate polar groups of analyte molecules for longer retention time. Does this affect…

A: HPLC is based on the principle where the sample (analyte) is distributed between a mobile phase…

Q: Are the number of spots shown on SDS-PAGE electrophoresis dependant on whether the protein is a…

A: It is to explain that whether the number of spots shown on SDS-PAGE electrophoresis is dependent on…

Q: Show calculations for making 1X TAE gel running buffer and 1.25% agarose gel for your experiment.

A: Buffer is a solution that tends to maintain pH of the solution when acid or base is added to it.…

Q: The first step in the procedure asks you to prepare five dilutions of the protein standard (BSA) in…

A: During the dilution process, solvent is added to the solution and hence changing the concentration…

Q: Given a disinfectant concentration dosage of 1.6 mg/L, estimate the retention time that you would…

A: The disinfectant concentration dosage of 1.6 mg/l Chlorine is used as a disinfectant. The…

Q: What are the different technical considerations in performing the serum protein electrophoresis…

A: Answer - The serum protein electrophoresis (SPEP) test measures specific proteins in the blood to…

Q: How is matrix selected in soft ionization? Give three examples.

A: Soft ionization is a type of ionization where a laser strikes a matrix of small molecules convert…

Q: What is the possible identity of the amino acid? [Select] What is the isoelectric point of AA?…

A: Since you have posted a question with multiple sub-parts, we will solve first three subparts for…

Q: C. Aminolysis: Anilide Formation Sample Color of precipitate formed Acetyl chloride Acetic anhydride

A: To tell the color of precipitate formed of anilide of acetyl chloride and acetic anhydride.

Q: 5. A scientist has a mixture of two peptides, A and B, and wants to separate them using ion exchange…

A: (a)   The resin is positively charged, i.e., anion-exchange resin.   So, we need a buffer where both…

Q: Explain the order of elution (with a buffer of pH 4) of the following pairs of amino acids through a…

A: (a)  Comparing the structure of aspartate and serine as shown. Aspartate has the carbonyl group…

Q: At pH 5.0? Q2) (a) Draw the structure of the predominant form (principal species) of…

A:

Q: Write with the reasons why a separation will occur when a mixture of polypeptides with isoelectric…

A: Isoelectric point (pI) is the pH value at which the positive charge equals the negative charge,…

Q: The pNa of a mixture prepared by mixing 200 ml of 0.00063 M of Nacl and 300 ml of 0.00040 M in…

A: The value of pNa is calculated by finding the concentration of Na+ ion in the mixture.

Q: What would be a suitable blank for an assay where the samples contain protein in a solution with dye…

A: A question based on absorption that is to be accomplished.

Q: Use the following chromatogram of standard amino acids: 24 16 19 21 12 15 6,6 9 10 23 13 14 17 18 10…

A: In chromatography, a higher retention time is linked to a higher affinity for the stationary phase…

Q: What is the Assay methods  of phenazopyridine?

A: Phenazopyridine is one of the drugs that is used to cure urinary tract infections. It is also used…

Q: The electrophoresis of a sample containing 2-amino-4-methylpentanoic acid. (pKa of a-COOH = 2.2, pKa…

A:

Q: You have a 10 mg/ml stock solution of ethidium bromide (EtBr) and need to dilute it to 0.2…

A:

Q: In what order would histidine, serine, aspartate, and valine be eluted with a buffer of pH 4 from a…

A: The order in which the following would the amino acids will be eluted with the buffer of pH 4 from a…

Q: Indicate the effect (increases, decreases, or stays the same) that each of the following changes…

A: A question based on analytical separations that is to be accomplished.

Q: A protein with a pI of 7.5 will be purified using ion-exchange chromatography.  Which ion exchange…

A: b)  A resin with sulfonic acid group is the correct option.

Q: Draw a titration curve for the tetrapeptide Trp-Asp-Lys-Gly. Label all pK,s, the pl and the net…

A: Trp-Asp-Lys-Gly

Q: Discuss the principles of the colorimetric and enzymatic methods for quantitating uric acid.

A: Generally there are two methods of quantitating uric acid . Colorimetric method  Enzymetic method

Q: Describe the difference between an oil-in-water and a water-in-oil emulsion, and explain the use of…

A: An emulsion is formed by combining two liquids which don't mix in to each other.

Q: What are the chief differences between the Ninhydrin and Bradford assays for amino acid…

A: Ninhydrin and Bradford assay both are performed to determine amino acids. Nin hydrin reacts with…

Q: Which of the following is not a component of the mobile phase in Ion exchange chromatography

A: Phase of mobility (Eluent)- Eluents for ion exchange chromatography are typically made up of an…

Q: Based on the graph, the retention of 4-aminobenzoic acid is not affected changes in mobile phase pH…

A: 4-amino benzoic acid remains in zwitterionic form in ph range 2-9 (approx) so on changing ph of…

Q: which of the following chromatographic methods is best used for separating amino acids?

A: Chromatography is the technique that is used for the separation of different components from the…

Q: Plot the titration curves of valine and lysine amino acids in basic medium and calculate the pI…

A: amino acids have ionisable group which ionizes at different condition depending on pH of the…

Q: While purifying a new protein using a dextran Sephadex, the consistency of the column material…

A: As per the bartleby expert guidelines, I am allowed to answer one question at a time .  Please…

Q: For two samples of cocaine, A was made up in an aqueous solution of pH 2.8, while B was made up in…

A: Given:-            The cocaine is the base due to tertiary amine group as a base. Therefore in…

Q: 3. Isoelectric focusing is a separation technique that takes advantage of the differences in…

A: Isoelectric focusing: It separates the different amino acids based on their differences in their pI…

Q: If prior to the analysis, the protein X needs to be solubilised in 250 mM acetate buffer, pH 4.0 to…

A:

Q: When analyzing a PTH (phenylthiohydratoin) derivative of the amino acid isoleucine, resulting from…

A: Reversed-Phase Chromatography: It is a liquid chromatography technique that involves the separation…

Q: titrimetric profile of lysine

A: Rough diagram

Q: what is The method that would be BEST to use to monitor protein secondary structure during the…

A: The secondary structure of the protein is affected by the complex folding properties of the protein…

Q: If 0.18 mL was used in the blank titration to achieve the same end-point color in the previous…

A: Hard water is the water sample that contains Ca2+ and Mg2+. Such water cannot be used in washing…

Q: what are the advantages of mediator-based amperometric enzyme sensors for glucose compred to…

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Q: Write one similarity between physisorption and chemisorption.

A: Physisorption is the physical adsorption process where as chemisorption is the chemical adsorption…

Q: 8. What properties cause the separation of amino acids in paper chromatography?

A: Different amino  acids have different partition coefficient between water and organic solvent.…

Q: The O2-consumption curve of a dilute, well-buff ered suspension of mito chondria containing an…

A: Mitochondria are centers for electron transport and oxidative phosphorylation. Reduced coenzymes…

Q: What are the physicochemical properties of the Poloxamer Gel? How do you compound 30 g of diclofenac…

A: In this we have to describe about the physicochemical properties of the Poloxamer Gel and the method…

Q: The peptide below is placed into a buffer with a pH = 1.54 -NH-CH-C HO- H;N-CH- CH2 -NH -CH-C…

A: We have to find out the direction of movement of peptide at pH=1.54