Answered: Write with the reasons why a separation…

Write with the reasons why a separation will occur when a mixture of polypeptides with isoelectric points 4,3, 8,7 and 9,2 is applied to a column containing an anion-exchanging filler.

Principles of Instrumental Analysis

7th Edition

ISBN:9781305577213

Author:Douglas A. Skoog, F. James Holler, Stanley R. Crouch

Publisher:Douglas A. Skoog, F. James Holler, Stanley R. Crouch

Chapter30: Capillary Electrophoresis, Electrochromatography, And Field-flow Fractionation

Section: Chapter Questions

Problem 30.3QAP

See similar textbooks

Related questions

Q: Write the differences between physisorption and chemisorption with respect to the following: (i)…

A: Physisorption is also called as physical adsorption. Chemisorption is adsorption process in which…

Q: How does the polarity of the amino acid affect its elution within the paper stationary phase?

Q: Look up the pKa values for trimethylamine, dimethylamine, methylamine, and ammonia. Predict the…

A: pKb tells about the basicity of any organic base. Higher the pKb value, lower would be the basicity.

Q: A weak acid, HA, has a pKa of 6.5. If you are utilizing ion-exchange chromatography with an anion…

A: ion-exchange chromatography is the technique used in Biomolecules such as Carbohydrates, protein…

Q: An amino acid mixture of phenylalanine, glycine and glutamic acid is to be separated bypaper…

A: As multiple questions are mentioned here, so the solution to the first part is given here. Paper…

Q: The amino acid cysteine typically gives two spots in TLC. Explain why this is so.

A: As amino acids are colourless ,to see the spots in chromatogram we apply a solution of ninhydrin to…

Q: The relative electrophoretic mobilities of a 30-kDa protein and a 92-kDa protein used as standards…

Q: Are the number of spots shown on SDS-PAGE electrophoresis dependant on whether the protein is a…

A: It is to explain that whether the number of spots shown on SDS-PAGE electrophoresis is dependent on…

Q: Which is correct for polarography? I-polarography is an amperometry method conducted with a dripping…

A: Polarography technique is used to determine the molecules are oxidation or other reduction at…

Q: Are the analyte molecules charged or not when isoelectric focusing separation is completed? Explain…

A: The analyte particles are charged. They just go to oppositely charged electrodes. Electrophoresis…

Q: Given a disinfectant concentration dosage of 1.6 mg/L, estimate the retention time that you would…

A: The disinfectant concentration dosage of 1.6 mg/l Chlorine is used as a disinfectant. The…

Q: If the biochemist used SDS-Polyacrylamide Gel Electrophoresis , predict the correct order of…

A: SDS-Page is used to separate a mixture of proteins having their mass in the range of 5 - 250 kDa.…

Q: The distribution coefficient for iodine between CCl4 and H2O is 85. Calculate the concentration of…

A: Given : Distribution coefficient (K) = 85 concentration of I2 = 1.00 x 10-3 Calculate the…

Q: A chromatography column has a stationary phase volume that is 0.130 times the volume of the mobile…

A: Given : The volume of stationary phase is 0.130 times than that of volume of mobile phase. Let the…

Q: Can be applied for the separation of ionic (nonprotein) analytes

A: Ans: IEX

Q: A sample containing histidine (pKas 1.8, 6.0, 9.3), lysine (pKas 2.2, 9.1, 10.5), and leucine (pKas…

A: Because a protein's net charge is pH-dependent, the sample may be eluted from IEX medium by varying…

Q: Use the following chromatogram of standard amino acids: 24 16 19 21 12 15 6,6 9 10 23 13 14 17 18 10…

A: In chromatography, a higher retention time is linked to a higher affinity for the stationary phase…

Q: Criteria and constraints help determine which solutions will be applied when extracting materials…

A: Many methods are used to extract materials from pomace.

Q: In what order would histidine, serine, aspartate, and valine be eluted with a buffer of pH 4 from a…

A: The order in which the following would the amino acids will be eluted with the buffer of pH 4 from a…

Q: Explain why the LC-MS-MS method is superior to the peptide mass mapping method.

A: Peptide mass fingerprinting (PMF) is an analytical technique for protein identification in which the…

Q: What are the basic medium, acid medium, and zwitterion formulas of phenylalanine?

A: Phenyl alanine is an amino acid. Amino acid exist in three form depending upon the pH of solution.…

Q: 5. What is number average and weight average molecular weight give equations with molecular weight…

A: Answer - Number-average - number average and weight average molecular weight is that the number…

Q: A protein with a pI of 7.5 will be purified using ion-exchange chromatography. Which ion exchange…

A: b) A resin with sulfonic acid group is the correct option.

Q: Describe the injection moulding process and distinguish between the injection moulding of TP and TS…

A: Injection moulding process is a method in which plastic materials are injected and are heated or…

Q: ttering technique. If the formulation of the subject sample has included surfactants or salt…

A: The size of small particles in solution or in suspension is determine by the light scattering…

Q: The standard curve of a biuret assay gave the equation: y=0.4193x. The absorbance for Unknown A =…

A: Biuret Protein Assay is a colorimetric technique specific to proteins and peptides. Biuret is a…

Q: NH3 obtained when 0.5 g of an organic compound substance was subjected to Kjeldahl's method was…

Q: What are the chief differences between the Ninhydrin and Bradford assays for amino acid…

A: Ninhydrin and Bradford assay both are performed to determine amino acids. Nin hydrin reacts with…

Q: For the determination of Pb in blood, a 5.00 mL sample was treated with trichloroacetic acid to…

A: The given data is as follows. A1 = 0.396 (first point) A2 = 0.599 (second point) C1 =…

Q: If a polystyrene sample gave a number average molecular weight of 300,000 Dalton and a…

A: Polydispersity index can be denoted by symbol PDI.

Q: 3. Isoelectric focusing is a separation technique that takes advantage of the differences in…

A: Isoelectric focusing: It separates the different amino acids based on their differences in their pI…

Q: Principles of separation by capillary Isolachophoresis

A: Capillary isotachophoresis: Capillary Isotachophoresis is a analytical chemistry technique which…

Q: In an experiment to measure the dipole moment of 1,2 dichlorobenzene, 1.603 g of this substance was…

A: Mole fraction = number of moles of the component / total number of moles of all the components…

Q: Describe the mechanism that allows analites to be separated in:ion-exchange chromatography

A: Interpretation - Describe the mechanism that allows analites to be separated in ion-exchange…

Q: Question attached

A: Paper chromatography is a qualitative method in order to detect the presence of colored components.…

Q: Write the significances of Poly Dispersity Index (PDI) and calculate the PDI for given data. Number…

A: Ploy Dispersity Index (PDI) tells us the distribution of molecular weights in a polymer. It is given…

Q: Describe the recent development, fundamental principle and application of micro contact printing in…

Q: A total of 5 mL of blood samples were treated with trichloroacetic acid to precipitate proteins.…

A: Absorbance of blood sample = 0.502 Absorbance of 0.4 ppm standard = 0.396 Absorbance of 0.6 ppm…

Q: titrimetric profile of lysine

A: Rough diagram

Q: The isoelectric point of tyrosine is 5.63. Toward which electrode does tyrosine migrate on paper…

A: Amino acid are the substances which have an amine group, a carboxyl group and a side chain which is…

Q: The relative electrophoretic mobilities of a 30-kDa protein and a 92-kDa protein used as standards…

A: The apparent mass of a protein can be calculated using the expression from the graph as given below:

Q: Briefly explain the principle behind the following techniques: (a) ion-exchange chromatography (b)…

A: Question is based on chromatography techniques:

Q: How does the shape of a titration curve confirm the fact that the pH region of greatest buffering…

A: As there are three question are present I answered only one. you can repost your remaining two…

Q: 24. Define a) chiral chromatography. b) adsorption chromatography.

A: As per rule we have answer only one question , so I am answering 24 question

Q: Why do most people elute bound proteins from an ion- exchange column by raising the salt…

A: Why do most people elute bound proteins from an ion- exchange column by raising the salt…

Q: If you had a mixture of proteins with different sizes, shapes, and charged and you separated them…

A: Gel electrophoresis is used to separate macromolecules like DNA, RNA and proteins. DNA fragments are…

Q: Name four factors (bonds or other forces) that contribute to stabilizing the native structure of a…

Q: Electrophoresis at pH 7.0 of the following lipid mixture is perfo lipid mixture…

Question

Write with the reasons why a separation will occur when a mixture of polypeptides with isoelectric points 4,3, 8,7 and 9,2 is applied to a column containing an anion-exchanging filler.

Expert Solution

This question has been solved!

Explore an expertly crafted, step-by-step solution for a thorough understanding of key concepts.

SEE SOLUTION Check out a sample Q&A here

Step 1

VIEW

Step 2

VIEW

Step by step

Solved in 2 steps

SEE SOLUTION Check out a sample Q&A here

Knowledge Booster

Learn more about

Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, chemistry and related others by exploring similar questions and additional content below.

Similar questions

Explain why a dispersion of silver nanoparticles coated with an amine functionalised polymer would be more stable at low pH than high pH.
Suppose two phosphate groups in DNA (each with a charge of 21) are separated by 12 Å. What is the energy of the ionic interaction between these two phos- phates assuming a dielectric constant of 80? Repeat the calculation assuming a dielectric constant of 2.
Two samples of polystyrene, samples A and B, were synthesised. A from the addition of n- butyl lithium to styrene in THF under a nitrogen atmosphere, and B from the addition of benzoyl peroxide to styrene under a nitrogen atmosphere respectively. 1. Of the two polymers produced (A and B) one had Ma= 12,000 and a polydispersity of 1.1 and one a Ma=11, 200 and a polydispersity of 1.7. Identify which set of molecular weight parameters corresponds to which polystyrene sample. %3D
For the amino acid alanine, the major species in solution at pH 7 is the zwitterionic form, which has a negatively charged carboxylic acid group and a positively charged amino group. There is a less common neutral form in which neither group is charged. The carboxylic acid group of alanine has a pK₁ of 3. The amino group of alanine has a pK₂ of 8. Estimate the ratio of the concentration of the neutral amino acid species to the zwitterionic species at pH 7. concentration ratio: Incorrect X10-3
Calculate the (a) Linearity constant (r), (b) y-intercept, (c) Slope, and (d) Protein content of an unknown sample having an absorbance of 0.325.
22-30. (a) The mean free path is the average distance a molecule travels before colliding with another molecule. The mean free path, A, is given by A = kT/(V20P), where k is Boltzmann’s constant, T is temperature (K), P is pressure (Pa), and o is the collision cross section. For a molecule with a diameter d, the col- lision cross section is nd. The collision cross section is the area swept out by the molecule within which it will strike any other molecule it encounters. The magnetic sector mass spectrometer is maintained at a pressure of ~103 Pa so that ions do not collide with (and deflect) one another as they travel through the mass analyzer. What is the mean free path of a molecule with a diam- eter of 1 nm at 300 K in the mass analyzer?
1. Calculate the Coulombic (ionic) interaction energy between atoms for the C=O ∙∙∙H-N hydrogen bond in a peptide at a distance of 1.5Å in units of kJ/mol. Assume that the partial charge on O is −0.434, the partial charge on H is +0.417 and the permittivity of the protein environment is 6. coulimbic interaction energy= 27.95 kJ/mol 2. The dipole moment of an individual peptide group is approximately 3.46 D. Assuming that the dipoles line up linearly estimate the energy of interaction (in kJ.mol) of the hydrogen bond in Q1 using a dipole-dipole model. *******please answer #2**********
22-49 Based on your knowledge of the chemical properties of amino acid side chains, suggest a substitution for leucine in the primary structure of a protein that would probably not change the character of the protein very much.
5. Given the following molecule glutathione (a tripeptide) determine the ionization states of the molecule at various pH's. The parts that are going to be affected by changing pH have pka values associated with them H3N pka = 10.0 pka = 2.2 OH Glutathione in a solution at pH 1 Glutathione in solution at pH 7 SH pka = 9.0 OH pka = 3.6
What are the advantages of a dropping-mercury electrode in polarography? Why is polarography used mainly to study reductions rather than oxidations?
a) 75mL of a 0.2M solution of a tripeptide Ser-Cys-His is adjusted to pH=8.6. The solution is then titrated with 50mL of 0.5M HCl. Calculate the new pH value of the tripeptide solution. (Relevant pKa values are: 2.2, 6.0, 8.3, and 9.6). b) Once you've completed the addition of HCl to the tripeptide in part a you then titrate the solution with 30 mL of 0.6M NaOH. Calculate the final pH value of the tripeptide solution.
a. Calculate the Coulombic (ionic) interaction energy between atoms for the C=O ∙∙∙H-N hydrogen bond in a peptide at a distance of 1.5Å in units of kJ/mol. Assume that the partial charge on O is −0.434, the partial charge on H is +0.417 and the permittivity(Er) of the protein environment is 6. b. The dipole moment of an individual peptide group is approximately 3.46 D. Assuming that the dipoles line up linearly estimate the energy of interaction (in kJ.mol) of the hydrogen bond in Q1 using a dipole-dipole model. please help me! this is for an upper division physical chemistry course, so showing work would be appreciated