Chemistry
Chemistry
10th Edition
ISBN: 9781305957404
Author: Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Publisher: Cengage Learning
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You need to separate a mixture of dyes on an agarose gel. You have been provided with 5X TAE (Tris acetate buffer) The electrophoresis tank needs 250 ml of buffer and the gel volume required is 50 ml. Show calculations for making 1X TAE gel running buffer and 1.25% agarose gel for your experiment. Explain how you will prepare the buffer and gel 4m

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