Show calculations for making 1X TAE gel running buffer and 1.25% agarose gel for your experiment.

Q: A solution with an ionic strength of 0.10M containing 0.0100M phenylhydrazine has a pH of 8.13.…

A: The objective is to determine the pKa of phenyhydrazine hydrochloride. Give Data:pH is 8.13.…

Q: Calculate buffer pH after adding strong acid or strong base. Determine the pH change when 0.063 mol…

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Q: of the solution. 4.00-mL of Na

A: Fully protonated form of tyrosine means solution will be in acidic form so use pka1

Q: For an enzymatic assay, tumour cells are incubated at different concentrations in individual vials…

A: The explanation is given below-

Q: 2. Complete the following table: pH 1.241 00 HI QZC4 100 mM anthoxanthin GB1 Ubiquitin -1.255 7.399…

A: pH of solution is given by the formula   ..........(1) pH < 7 solution is acidicpH > 7…

Q: Fig. 1 shows the possible effect of pH on recovery and impurity removal at the selection of a…

A: The Y-axis of the graph represents the percent recovery of the impurity, and the X-axis of the graph…

Q: Which is correct for polarography? I-polarography is an amperometry method conducted with a dripping…

A: Polarography technique is used to determine the molecules are oxidation or other reduction at…

Q: True or False An essential amino acid is one which is not synthesized in the body and thus must be…

A: 1.) An essential amino acid is one which is not synthesized in the body and thus must be supplied in…

Q: Copper is known to be toxic to fish, and in particular, the Cu²+ species shows greatest toxicity. a.…

A: In the given question we have to calculate the speciation of Cu(II) for freshwater as the hardness…

Q: You decided to take the pH after each addition of 5.0 mL of NaOH rather than after every 0.5 mL…

A: With the help of this equation, pH and pka of buffer solution can be calculated. pH = pKa + log…

Q: Istandard hard water (containing 15gms of CaCO3 per 500 mL) required 25 mL of EDTA Solution for…

A: The question is based on the concept of complexometric titrations. we determine the hardness of the…

Q: Make 50 ml of the extraction buffer containing the following ingredients using the stock solution…

A: We need to make 50 mL of the extraction buffer.

Q: 5. A scientist has a mixture of two peptides, A and B, and wants to separate them using ion exchange…

A: (a)   The resin is positively charged, i.e., anion-exchange resin.   So, we need a buffer where both…

Q: 1. You have been provided with the following solution: 0.2M NaOH and 0.2M acetic acid How much…

A: We are given with a solution of 0.2M NaOH and 0.2M acetic acid solution. We need to find how much…

Q: Your titration experiment included phosphoric acid, liqid starch, and Iodine-Potassium-Iodide (IKI)…

A: To address this Q, we must first establish as to what are primary and secondary…

Q: 2. Predict the solubilities, either organic of mixture of ethyl acetate and water (pH 7). What…

A: Dye is a colored compound due to the presence of chromophore and its fixed property to the acid or…

Q: i Look up and list the size of the following proteins; then predict in what order they would be…

A: Soln Compounds elute in order of decreasing size in Gel Filtration Chromatography. This is due to…

Q: I was just wondering how you got the 4.0 x 10-4 for the acidionzation constant.

A: Given for the 0.500 M HNO2  HNO2 = nitrous acid  It dissociates into H+ ion and NO2- ion .

Q: You want to use acid-base extraction to separate compound A (pKa = 4.9) from compound B (pKa = 9.5).…

A: Based on the pKa values of compounds A and B, it is evident that compound A is more acidic than…

Q: You prepare a buffer solution from 10.0 mL of 0.100 M MOPS (3-morpholinopropane-1-sulfonic acid) and…

A: A buffer is a solution that can resist pH change upon the addition of an acid or base. It is able to…

Q: A solution containing a mixture of metal cations was treated as outlined. 1. Dilute HCI was added…

A: What can be said about the presence of each of these groups of cations in the original solution?

Q: The method for determining the concentration of nitrate and nitrite using capillary…

A: A multiple choice question based on analytical process that is to be accomplished.

Q: In a solution containing a mixture of the amino acids glutamic acid, lysine and serine the pH is…

A: The pH is adjusted to 5.68The mixture contains glutamic acid, lysine and serine

Q: Which way will negatively charged molecules migrate?   in the opposite direction of the current…

A: In the electrolysis the movement of particle is based on the charge of molecule. If the molecule is…

Q: ind the ka and kb of acetic acid at 0.2 M from the experimental pH range of 1.2-2.8 and compare your…

A: Given that , experimental range of pH for acetic acid is, pH =1.2-2.8 Concentration of acetic acid…

Q: Copper is known to be toxic to fish, and in particular, the Cu²+ species shows greatest toxicity. a.…

A: To calculate the speciation of Cu(II) in freshwater at a pH of 8.3 as the hardness increases, we…

Q: the percentage of sodium bicarbonate in the sample

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Q: n RPLC, The retention of a basic analyte in RPLC decreases as the mobile phase pH increases. True or…

A: Silica gel and alumina are highly polar so they interact strongly with polar compounds and solvent…

Q: What would your main concern be if you accidentally used wash buffer (10mM imidazole) instead of…

A: Answer is explained below.  We know that, for binding to the metal-charged resin Imidazole competes…

Q: You run a DEAE-cellulose column to purify anions from a biological sample. Which approach, from the…

A: We are running the column chromatography of DEAE-cellulose to separate anions. We are trying here to…

Q: Find the Ksp of  distilled water with Mg(OH)2 as its salt (solubility:1.51121E-05 M) MgCO3 as its…

A: The equilibrium between the soluble ions and insoluble salt can be described by the solubility…

Q: 2. If 6 g of phenol (weak acid, pKa 9.9) is distributed between 100 mL of an aqueous buffered…

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Q: 1. “Using your 100 units/mL extract, prepare 20 mLs of extract at concentrations of 75, 50,and 25…

A: The volume (V) of 100 units/mL extract required to prepare 20 mL of concentration of 75 units/mL…

Q: Below is overlaid chromatograms for the separation of cytidine on a bare silica stationary phase…

A: The time period for which a solute is present in the column for the interaction with the stationary…

Q: The following data are the results of an experiment in which 0.5mL aliquots of invertase solutions…

A: The objective is to determine the correct statement regarding invertase activity.Given:Temperature…

Q: 5. Say you'd like to assess the performance of an aqueous reaction at pH 6 and pH 8.2. 1. Which…

A: A buffering agent is chosen in such a way that the pKa of the buffer should be near the desired pH…

Q: Calculate gram quantity of Tris and the volume of HCl to prepare 100mL of 1 M Tris Buffer at pH 7.5.…

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Micelles and Biological Membrane

Micelles are the type of lipid molecules that organize themselves in spherical shape in aqueous solutions. The production of the micelle is a reply to the amphipathic behavior of fatty acids. It means that they consist of both hydrophobic regions and hydrophilic regions. In micelles, polar head groups (hydrophilic groups) produce an outer layer associated with water whereas nonpolar tails (hydrophobic groups) are organized in the interior.