What are the 3 R's in animal experiments? Explain each.

paper chromatography:   1.If the Rf of blue was 0.81, Rf of yellow was 0.68, and Rf of red was 0.64, then which color would be the heaviest? which one would be the lightest? and why? Gel electrophoresis:   2.Given that well 1 represented protein from a dog (Rf=0.45), well 2 from a wolf (Rf=0.73) and well 3 from a fox (Rf=0.76), and the unknown well represented protein from a "chupacabra". what animal would say the "chupacabra" is closely related to?   3.which one would be the heaviest and lightest in given above electrophoresis? and why?

How would you calculate the expected values from the observed in order to use the chi-squared test?

What would be the central dogma of biology? Explain it briefly?

What is PEI and what is its purpose in this protocol?

What might Watson and Crick have concluded had Chargaff’s datafrom a single source indicated the following? A T G C % 29 19 21 31 Why would this conclusion be contradictory to Wilkins’s and Franklin’s data?

can you explain for me the lead time bias and length bias ?

What did Rosalind Franklin, Maurice Wilkins, Francis Crick, and James Watson discover that resulted in the Nobel Prize being awarded to all of them except Franklin (Franklin was ineligible because she was deceased)?

Plate LB-Plasmid LB + Plasmid LB/amp - Plasmid Yes Yes No + LB LB/amp + Plasmid Yes LB Prediction Reeson Plate only contains Yes food/bacteria Plate only contains Yes food/bacteria Bacteria will be killed by ampicillin Bacteria has ampicillin resistance LB Click on pic to zoom Click on pic to zoom -LB Observed Result No Yes chlamp x Click on pic to zoom + LB/AMP Blamp Click on pic to zoom - LB/AMP

What was studied or investigated in the paper? Who would be affected by this? https://opus.lib.uts.edu.au/bitstream/10453/31887/1/2012007630OK.pdf