Aside from H&E, what other staining methods are applicable in Golgi apparatus, mitochondria, and nucleic acid? What are the differences?

Q: Why is the order of separation based on size opposite for gel filtration and gel electrophoresis,…

A: Gel filtration and gel electrophoresis are biochemical techniques used for the separation of…

Q: Ethidium bromide used to be a common stain for detecting DNA/ RNA on agarose gel. (i) Describe how…

A: Ethidium Bromide is a DNA stain as well as a mutagen. This dye can be visualized under UV light. It…

Q: Explain the mechanisms of the four types of fluorescence microscopy we discussed in lab: 1) GFP…

A: A fluorescence microscope is an optical microscope that studies the characteristics of organic or…

Q: The diffraction limit describes the limitationof light microscopes to distinguish objects less than…

A: Combining image gathering with computationally intensive image analysis can yield a wealth of…

Q: What is the significance of UV unwrapping in the texturing process of 3D models?

A: It plays a pivotal role in the process of texturing 3D models. It includes applications in the field…

Q: Since higher concentration colcemid will result in shorter chromosome, you want to      change your…

A: It is given that: Molar concentration of the stock solution: 10 ug/ml.  Desired dilution…

Q: What are the main characteristics of scanning electron microscopy?

A: Electrons are the negatively charged particles having minus 1 charge  and represented by e- ,…

Q: What are the advantages and disadvantages of light, transmission, and scanning electron microscopes?

A: Microscopy is the primary or initial technology to observe microscopic substances and organisms, and…

Q: For a stain to be considered differential, what must it do?

A: Differential stain uses more than two stains.

Q: What is the charge of the target cellular structure of positive staining and negative staining?

A: Basic stains are cationic when it is ionized, the chromogen exhibits a positive charge. the positive…

Q: How could you isolate mitochondria from liver cells using differential centrifugation?

A: Isolated mitochondria are used to perform several functions. This organelle is used to study…

Q: high-resolution image in an optical microscope?

A: Resolution is the fineness and detailing of the picture and it is measured in pixels per inch…

Q: Why should there be 3 banding techniques (G, Q, and R)? Aside from their differences in appearance…

A: Banding techniques are utilized to understand the staining effects in the chromosomes so that the…

Q: What is the purpose of compound light microscope? What is a compound light microscope and where did…

A: A microscope is a device to observe objects that cannot be seen with naked eyes or viewing finer…

Q: Give three situations where negative staing procedure would be used?

A: Negative staining is a staining technique in which the background is stained whereas the specimen…

Q: What is a differential stain? why is it used?

A: Differential stain is a type of stain use to identify organisms.

Q: What is a wet mount technique in microscopy?

A: Microscopy is the technical field of using microscopes to view samples and objects that the naked…

Q: Ethidium bromide used to be a common stain for detecting DNA/ RNA on agarose gel. (i) Describc how…

A: During the extraction of DNA fragments via agarose gel electrophoresis, EtBr or ethidium bromide is…

Q: Can two-dimensional gel electrophoresis be used as a purificationtechnique? Explain.

A: Gel electrophoresis is a laboratory technique, which is used to separate mixtures of DNA or RNA…

Q: What do you mean by fluorescent-labeled probes?

A: Fluorescent marking is the interaction of covalently binding fluorescent colors to biomolecules,…

Q: a) Make a drawing of the cells from each culture to identify the various stages of the cell…

A: Saccharomyces cerevisiae is a eukaryotic organism that is used in various scientific experiments. It…

Q: How can agarose gel electrophoresis process be imporved? What are ways to do it?

A: Electrophoresis means the migration of a charged particle under the influence of an applied electric…

Q: What is the difference between conventional optical microscopes and inverted microscopes?

A: Introduction Conventional optical microscopes:- This type of microscope uses visible light (400-700…

Q: Explain two-dimensional gel electrophoresis in detail.

A: Proteins are the building blocks of life. Proteins are made up of amino acids. Amino acids when they…

Q: Why must materials and instruments used in tissue culture be kept sterile?

A: Sterilization of explants is a key step in plant tissue culture.

Q: What can be done with the food dye to utilize it as an additive biocompatible material to improve…

A: 3D bioprinting offers great potential to fabricate highly complex constructs with precise control of…

Q: What is a microscope? What is its significance in the Medical Technology profession?

A: Microbiology is the study of microorganisms, which are unicellular or cell-cluster microscopic…

Q: microscopic study of mitosis in plants, what basic preparations

A: Plant mitosis (equational cell division) is very similar to that of animal mitosis. First two phases…

Q: Immunofluorescence and Fluorescent live cell imaging techniques can both be used to determine…

A: Immunofluorescence is a technique in which fluorochrome labeled-antibodies are used to detect the…

Q: What is the purpose of adding two drops of acetic acid in thin layer chromatography of plant…

A: Plant pigments are natural substances found in plant cells that give color to different parts of the…

Q: 4- Mitotic spindle is observed with: a) O Keratin filaments b) O Actin filaments C)O Cytoplasmic…

A: The growth and development of organisms are attributed to the unique ability of the cell to divide…

Q: Which pigment spots (or bands) produce fluorescence under long-wave ultraviolet light?

A: A pigment is an element that imparts colour to a substance or surface by preferentially absorbing or…

Q: What is the difference and compare the light source and path of light of a immunofluorescence…

A: Introduction  : A microscope is a tool that can be used to examine cells and other tiny objects.…

Q: What type of probes must be used for super-resolutionmicroscopy and why?

A: The super-resolution microscopy involves a series of techniques that allow high-resolution images.…

Q: In your opinion, for efficient multiphoton excited fluorescence microscopy, would it be better to…

A: The absorption and subsequent re-radiation of light by organic and inorganic specimens is typically…

Q: What is the secondary stain in the spore stain?

A: Endospores are the structures that are formed by bacteria during unfavorable conditions (lack of…

Q: You wish to identify the presence of a specific protein inside the cell. What microscopy technique…

A: Microscopy is a scientific technique that involves the use of microscopes to observe and study…