True'and false please with brief explanation. True or False1. A buffer composed of a weak acid ofpKa = 6 is stronger at pH 5 than at pH%3D7.2. At pH values higher than the pKa, thesalt concentration is higher than that ofthe acid.3. The pH of a buffered solution remainsconstant no matter how much acid orbase is added to the solution.4. The strongest buffers are thosecomposed of weak acids and weakbases (not strong).5. When pH = pKa, the weak acid and saltconcentrations in a buffer are not equal.

1.False-A weak acid/base best buffers about 1pH point above and below its pKa. 2.True. If the pH of…

Answered: True'and false please with brief…

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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7. · In the space provided, sketch a titration curve (pH vs H+ ions dissociated) of a phosphate buffer shown below. The pKa of H2PO4 is 7.0. H,PO, НРО, + 9. 8. 7 pH 3 1.0 0.5 H+ dissociated --> b. What is the effective buffering range of this acid? At what pH will you have 25% of the buffer in the form of H2PO4? с.
Choose the FALSE statement An indicator can be used to monitor a buffer range When pH equals PKa of weak acid, the buffer capacity is the greatest Polyprotic acids have multiple stoichiometric points in their titration curve At the mid-point of titration of weak acid by strong base, the pH of the solution -does not equal the pKa of the weak acid The moles of titrant equals moles of titrate
Part I. For each of the following four questions: calculate and describe how the requested solution would be made. Please show all of your calculations. In all cases the diluent or solvent will be water. Also, assume the smallest volume you can accurately pipette is 1ul. (CaClh: Molecular weight = 111.0 - NaCl: Molecular weight= 58.44) 1. From a 3M CaCh stock solution, you need to make 600 ml of 9 mM CaClh. Indicate how you would make it. 2. You have a 20ul sample of DNA that you want to run in a gel. You are given 5X track dye; how much track dye do you add to your sample so that the track dye concentration in the sample is 1X? 3. You need to make 6 liters of 20% NaCl solution. Indicate how you wvould make it firom powdered NACI.
. An acetate/acetic acid (pKa=4.75) buffer of 500 mL includes 0.1 mol of acetate and 0.5 mol of acetic acid. 2.0 M NaOH was added to the buffer to get the pH to 4.70. What volume of NaOH was added to the system?
An appropriate biochemical buffer should have charges in both its conjugate acid and conjugate base form. This avoids the presence of a neutral form of the buffer that might be able slowly leak into cells. Does unmodified dimethylpiperazine meet this criterion when buffering at pH 8? O Yes No What about buffering at pH 4? D Yes No
pH with 0 mL of pH with 5 mL of pH with 10 mL of Cola Buffer pH with 20 mL Cola Cola of Cola Buffer F 4.5 4.8 4.1 4.4 Buffer H (water) |7.0 3.2 n/a n/a Is Buffer F (the acetic acid - sodium acetate buffer you made) a good buffering system for acids? Explain your reasoning.
Define the term buffer capacity and explain if the amino acid phenylalanine would be a good buffer at pH 9. Motivate your answer in detail.
Blood is an alkaline buffer having pH around 7.4. If blood would not be a buffer, we may not survive”. Justify this statement with alkaline buffer mechanism in three sentences.
Concentrations and volumes are NOT the same thing. Concentrations are in units of mass/volume (i.e., g/l or mg/ml). Volumes are in units of only volume (i.e., 1. ml). 4. a. If you mixed 265 g of sugar with water to get a final volume of 725 ml. what is the concentration of this solution in g/l? Show your work. If you took 1 ml of this solution, what would the concentration be? Explain your reasoning. you prepare a stock solution (highly concentrated) of sugar in water whose concentration is 500 g/l. you need to make a new solution that is half (1/2) as concentrated and has a final volume of 100 ml. Describe how you would make this dilution. Step one: Your new solution has a concentration of_. Step two: To make 100 ml of this new solution, you will need to add_of concentrated stock solution plus_of water. Now make a second solution that is one-tenth (1/10) as concentrated as the initial stock solution and has a final volume of 100 ml. Describe how you would make this dilution.…
OSTATES OF MATTER Finding mass or volume from percent concentration To my home Com GED Math Pract Ketamine, sometimes used as an adjuvant to general anesthesia, is available as a 1.0 %(w/v) solution. Calculate the volume of this solution that contains 20 mg of ketamine. Be sure your answer has a unit symbol and is rounded to the correct number of significant digits.
I have this practise question and hav no idea where to start. Please help. You are supplied with the following: NaCl (Mr = 58.443 g/mol) 2.5 M Tris-Cl, pH 8 oplossing / solution (1 Litre) EDTA, sodium salt (Mr = 380.2 g/mol) 10 % Sodium dodecyl sulfate solution Proteinase K solution (50 mg dissolved in 1 ml ddH2O) You need a digestion buffer consisting of the following: 15 mM NaCl 75 mM Tris-Cl, pH 8 16 mM EDTA, pH 8 0.8 % Sodium dodecyl sulfate 0.75 mg/ml proteinase K How will you prepare 500ml of digestion buffer? Show all the steps.
Calculate the volume of TAE buffer that you will need to prepare a 100ml solution of 1X strength if you only have a 50X concentrated solution available.

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