Part I. For each of the following four questions: calculate and describe how the requested solution would be made. Please show all of your calculations. In all cases the diluent or solvent will be water. Also, assume the smallest volume you can accurately pipette is 1ul. (CaClh: Molecular weight = 111.0 - NaCl: Molecular weight- 58.44) 1. From a 3M CaCh stock solution, you need to make 600 ml of 9 mM CaCl2. Indicate how you would make it. 2. You have a 20ul sample of DNA that you want to run in a gel. You are given 5X track dye; how much track dye do you add to your sample so that the track dye concentration in the sample is 1X? 3. You need to make 6 liters of 20% NaCl solution. Indicate how you would make it from powdered NaCl.

Concentrations and volumes are NOT the same thing. Concentrations are in units of mass/volume (i.e., g/l or mg/ml). Volumes are in units of only volume (i.e., 1. ml). 4. a. If you mixed 265 g of sugar with water to get a final volume of 725 ml. what is the concentration of this solution in g/l? Show your work. If you took 1 ml of this solution, what would the concentration be? Explain your reasoning. you prepare a stock solution (highly concentrated) of sugar in water whose concentration is 500 g/l. you need to make a new solution that is half (1/2) as concentrated and has a final volume of 100 ml. Describe how you would make this dilution. Step one: Your new solution has a concentration of_. Step two: To make 100 ml of this new solution, you will need to add_of concentrated stock solution plus_of water. Now make a second solution that is one-tenth (1/10) as concentrated as the initial stock solution and has a final volume of 100 ml. Describe how you would make this dilution.…

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