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Part I. For each of the following four questions: calculate and describe how the requested solution would be made. Please show all of your calculations. In all cases the diluent or solvent will be water. Also, assume the smallest volume you can accurately pipette is 1ul. (CaClh: Molecular weight = 111.0 - NaCl: Molecular weight- 58.44) 1. From a 3M CaCh stock solution, you need to make 600 ml of 9 mM CaCl2. Indicate how you would make it. 2. You have a 20ul sample of DNA that you want to run in a gel. You are given 5X track dye; how much track dye do you add to your sample so that the track dye concentration in the sample is 1X? 3. You need to make 6 liters of 20% NaCl solution. Indicate how you would make it from powdered NaCl.

Part I. For each of the following four questions: calculate and describe how the requested solution would be made. Please show all of your calculations. In all cases the diluent or solvent will be water. Also, assume the smallest volume you can accurately pipette is 1ul. (CaClh: Molecular weight = 111.0 - NaCl: Molecular weight- 58.44) 1. From a 3M CaCh stock solution, you need to make 600 ml of 9 mM CaCl2. Indicate how you would make it. 2. You have a 20ul sample of DNA that you want to run in a gel. You are given 5X track dye; how much track dye do you add to your sample so that the track dye concentration in the sample is 1X? 3. You need to make 6 liters of 20% NaCl solution. Indicate how you would make it from powdered NaCl.

Biochemistry
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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Part I. For each of the following four questions: calculate and describe how the requested solution would be made. Please show all of your calculations. In all cases the diluent or solvent will be water. Also, assume the smallest volume you can accurately pipette is 1ul. (CaClh: Molecular weight = 111.0 - NaCl: Molecular weight= 58.44) 1. From a 3M CaCh stock solution, you need to make 600 ml of 9 mM CaClh. Indicate how you would make it. 2. You have a 20ul sample of DNA that you want to run in a gel. You are given 5X track dye; how much track dye do you add to your sample so that the track dye concentration in the sample is 1X? 3. You need to make 6 liters of 20% NaCl solution. Indicate how you wvould make it firom powdered NACI.
Transcribed Image Text:Part I. For each of the following four questions: calculate and describe how the requested solution would be made. Please show all of your calculations. In all cases the diluent or solvent will be water. Also, assume the smallest volume you can accurately pipette is 1ul. (CaClh: Molecular weight = 111.0 - NaCl: Molecular weight= 58.44) 1. From a 3M CaCh stock solution, you need to make 600 ml of 9 mM CaClh. Indicate how you would make it. 2. You have a 20ul sample of DNA that you want to run in a gel. You are given 5X track dye; how much track dye do you add to your sample so that the track dye concentration in the sample is 1X? 3. You need to make 6 liters of 20% NaCl solution. Indicate how you wvould make it firom powdered NACI.
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