Homogenize cells Centrifuge Heat-kill Recover IIIS filtrate IIIS cells spun to bottom of tube Extract carbohydrates, lipids, and proteins IIIS cells in liquid culture medium Treat with Treat with ribonuclease Treat with deoxyribonuclease protease Assay for Transformation IIR cells IIR cells IIR cells IIR cells + + IIIS filtrate DNase-treated IIIS filtrate RNase-treated IIIS filtrate Protease-treated IIIS filtrate No transformation Transformation Transformation Transformation occurs occurs occurs occurs

Homogenize cells Centrifuge Heat-kill Recover IIIS filtrate IIIS cells spun to bottom of tube Extract carbohydrates, lipids, and proteins IIIS cells in liquid culture medium Treat with Treat with ribonuclease Treat with deoxyribonuclease protease Assay for Transformation IIR cells IIR cells IIR cells IIR cells + + IIIS filtrate DNase-treated IIIS filtrate RNase-treated IIIS filtrate Protease-treated IIIS filtrate No transformation Transformation Transformation Transformation occurs occurs occurs occurs

Principles Of Pharmacology Med Assist

6th Edition

ISBN:9781337512442

Author:RICE

Publisher:RICE

Chapter15: Antibiotic Agents

Section: Chapter Questions

Problem 19RQ

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A PhD student leaving for vacation has asked an undergraduate student to perform daily media changes forhis iPSCs while he is gone. The culture is happening in 12-well plates where a volume of 2 mL is optimal. OnSaturday, when changing the media, the undergraduate decides to add 4 ml of media to the dishes (insteadof 2 ml) because he wants to skip lab and watch the Super Bowl on Sunday. He decides to add twice thevolume of media (4 mL) to tide the cells over till Monday. However, when the graduate student returns onMonday, he finds that some of his cells have died. Your job is to determine whether the cells died due to alack of oxygen. For the calculations that follow, diffusion and reaction occurs only in one direction. Also,assume that reaction only occurs at the cell-media interface. Use Michaelis-Menten type kinetics for oxygenuptake. You may use the following information:Ps = 150 mmHg (ambient oxygen tension)K = 1.19 nmol / mL / mmHg (solubility of oxygen in medium)D = 2…
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