Briefly explain partition coefficients. How is this concept applied in biotechnology?

4). Mutants unable to synthesize an essential compound G were tested with related compounds D, E, and F. The results are summarized below, where + indicates growth, and 0 indicates no growth. Mutants 1 2 3 4 D 0 0 0 Compounds E 0 INAAL F 0 0 INAY a). What is the order of gene action in the biosynthetic pathway? G

Despite the failure to date of gene therapy, identification of the gene responsible for cystic fibrosis has very recently led to effective treatments for the disease in patients with particular mutant alleles. Give an example?

Both the isolation buffer and assay buffer that was used in isolation of mitochondria contained 0.3M mannitol. What was the purpose of including mannitol at this concentration?

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Who revealed biochemical nature of the transforming principle?how was it done?

28. In a transformation experiment, DNA is collected from an E. coli donor strain of genotype cys(+) leu(-) thr(+) and used to transform a recipient of genotype cys(-) leu(+) thr(-). Initially, the treated recipient population is plated on a minimal medium supplemented with threonine. Many colonies are obtained. What are possible genotypes of these colonies? A. cys(-) leu(+) thr(+) B. cys(+) leu(+) thr(-) C. All are cys(+) and either + or - for leu and thr D. All are thr(+) and either + or - for cys and leu E. All are thr(-) cys(-) and either + or - - for CYS

Scientists are currently screening a chemical library of small molecules for inhibitors of bioluminescence in response to high cell density in V. fischeri.The small molecules were chosen for their potentialability to bind LuxR.a. How could a molecule that binds LuxR preventbioluminescence?b. Hundreds of different bacterial species use quorumsensing mechanisms similar to that of V. fischeri andencode proteins similar to LuxI and LuxR. In lightof this information, why do you think scientists wantto identify the molecule described in part (a)?

Select all that apply from the following to each transformation process : The following are White non-glowing colonies White glowing colonies Blue non-glowing colonies Blue glowing colonies None of the above The transformation are: a. LB + kanamycin plates that have been spread with the E. coli cells transformed with your ligations, what phenotype of the colonies do you expect to obtain . b. LB + kanamycin plates that have been spread with the E. coli cells transformed with water, what phenotype of the colonies do you expect to obtain. c. LB plates that have been spread with the E. coli cells transformed with your pHSG298, what phenotype of the colonies do you expect to obtain. d. LB + kanamycin plates that have been spread with the E. coli cells transformed with PHSG298, what phenotype of the colonies do you expect to obtain. e. LB plates that have been spread with the E. coli cells transformed with your ligations, what phenotype of the colonies do you expect to obtain. f. LB plates…