TRUE FALSEEF-Ts factor regenerates EF-Tu/GDP from EF-Tu/GTP for thenext round of elongation cycle in prokaryotic translation.The sigma o subunit of RNA polymerase is replaced by NusA torecognise the promoter region of a gene.The -10 and -35 sequences in E. coli DNA are referred to asuntranslated region.In human, translation begins before transcription of an mRNAcompletes.The distal histidine stabilises the iron in heme group of adeoxyhaemoglobin.Receptor for epinephrine consists of seven transmembranehelices.Membrane receptors bind to hydrophobic signaling molecules.Amino acids, glucose and fatty acids are taken up by transportersinto liver cells.Glycerol and fatty acids are synthesized from the intermediatesof glycolysis.Synthesis of fatty acid occurs in cytoplasm.Triacylglycerol can be obtained from diet and also synthesized inliver cells.Ammonia and keto acid are formed in transamination.

There are many biomolecules present in an organism and each biomolecule has a specific role.…

TRUE FALSE EF-Ts factor regenerates EF-Tu/GDP from EF-Tu/GTP for the next round of elongation cycle in prokaryotic translation. The sigma o subunit of RNA polymerase is replaced by NusA to recognise the promoter region of a gene. The -10 and -35 sequences in E. coli DNA are referred to as untranslated region.

TRUE FALSE EF-Ts factor regenerates EF-Tu/GDP from EF-Tu/GTP for the next round of elongation cycle in prokaryotic translation. The sigma o subunit of RNA polymerase is replaced by NusA to recognise the promoter region of a gene. The -10 and -35 sequences in E. coli DNA are referred to as untranslated region.

Biochemistry

6th Edition

ISBN:9781305577206

Author:Reginald H. Garrett, Charles M. Grisham

Publisher:Reginald H. Garrett, Charles M. Grisham

Chapter29: Transcription And The Regulation Of Gene Expression

Section: Chapter Questions

Problem 16P

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EF-Ts factor regenerates EF-Tu/GDP from EF-Tu/GTP for the next round of elongation cycle in prokaryotic translation. The sigma subunit of RNA polymerase is replaced by NusA to recognise the promoter region of a gene. The -10 and -35 sequences in E. coli DNA are referred to as untranslated region. In human, translation begins before transcription of an mRNA completes. this statement true or false.
The RNA polymerase from bacteriophage T7 diff ers structurally from prokaryotic and eukaryotic RNAPs and is extremely specifi c for its own promoter. Why do these properties make T7 RNAP useful in experiments with recombinant DNA?
All eukaryotes possess a surveillance pathway referred to asnon-sense-mediated mRNA decay (NMD). Its principalfunction is to eliminate mRNA transcripts with prematurestop codons. Such faulty transcripts are detected duringtranslation and subsequently destroyed by removal of the 5′cap followed by degradation by a nuclease. Describe howpremature stop codons are detected and what type of errorcauses them.
Compared to heterochromatin, euchromatin is comprised of densely packed nucleosomes and with higher transcription activity comprised of densely packed nucleosomes and with lower transcription activity O comprised of loosely packed nucleosomes and with lower transcription activity Ocomprised of loosely packed nucleosomes and with higher transcription activity
During translation, the tRNA antlicodon sequence G-A-U vyould blnd to which MRNA codon (plck one of the cholces I -V below)? Note: all of the sequencos for tho quostlon and answors use the standard convention for representing ollgonuclootidos discussed In class whoro tho 5'-ond Is at the loft and the 3'-ond Is at the right. I) G-A-U II) U-A-G I) C-U-A IV) A-U-C V A-T-C OA. none of the cholces OB. IV Oc." OD.! OE, II
Arrange the following components of translation in the approximate order in which they would appear or be used in prokaryotic protein synthesis: 70S initiation complex 30S initiation complex release factor 1 elongation factor G initiation factor 3 elongation factor Tu fMet-tRNAi fMet
The Events in Transcription Initiation Describe the sequence of events involved in the initiation of transcription by E. coil RNA polymerase. Include in your description those features a gene must have for proper recognition and transcription by RNA poIymerase.
Substrate Binding by RNA Polymerase RNA polymerase has t binding sites for ribonucleoside triphosphates: the initiation site and the elongation site. The initiation site has a greater Km for NTPs than the elongation site. Suggest what possible significance this fact might have for the control of transcription in cells.
Given the following tRNA anticodon sequence, derive the mRNA and the DNA template strand. Also, write out the amino acid sequence of the protein encoded by this message. tRNA: UAC UCU CGA GGC mRNA: protein: How many hydrogen bonds would be present in the DNA segment?
A normal mRNA that reads 5’ - UGCCAUGGUAAUAACACAUGAGGCCUGAAC- 3’ has an insertion mutation that changes the sequence to 5' -UGCCAUGGUUAAUAACACAUGAGGCCUGAAC- 3’. Translate the original mRNA and the mutated mRNA, and explain how insertion mutations can have dramatic effects on proteins. (Hint: Be sure to find the initiation site.)
Post-transcriptional Modification of Eukaryotic mRNAs What might be the advantages of capping, methylation, and polyadenylylation of eukaryotic mRNAs?
The Lariat Intermediate in RNA Splicing Draw the structures that comprise the lariat branch point formed during mRNA splicing: the invariant A. its 5â€™-R neighbor, its 3â€™-Y neighbor, and its 2â€™-G neighbor.