transferred to *"*N-containing "light" medium for one cell division. The results of this experiment ruled out the "conservative" model of DNA replication, but to distinguish between the "semiconservative" model and the "dispersive" model, the DNA had to be heated to separate the two strands. You are repeating the experiment, but are unable to heat the DNA to perform the second part. However, you realize that allowing the bacteria to undergo a second round of division would also distinguish between the different models. Which of the following would you expect to see as a result after the second division in "light" medium? Choose one: O A. After the second division, one population of DNA would run at the same position as DNA from bacteria grown fully in "light" medium, and one population would run at a position halfway between fully "heavy" and fully "light" DNA. B. After the second round of replication, all of the DNA would run at a position intermediate between fully "heavy" DNA and fully "light" DNA, but closer to the "light" DNA than seen after the first round of replication (i.e., 3/4 "light"). O C. After the second round of replication, all of the DNA would run at a position halfway between fully "heavy" DNA and fully "light" DNA. D. After the second round of replication, 25% of the DNA would run at a position similar to fully "heavy" DNA and 75% would run at a position similar to fully "light" DNA.
transferred to *"*N-containing "light" medium for one cell division. The results of this experiment ruled out the "conservative" model of DNA replication, but to distinguish between the "semiconservative" model and the "dispersive" model, the DNA had to be heated to separate the two strands. You are repeating the experiment, but are unable to heat the DNA to perform the second part. However, you realize that allowing the bacteria to undergo a second round of division would also distinguish between the different models. Which of the following would you expect to see as a result after the second division in "light" medium? Choose one: O A. After the second division, one population of DNA would run at the same position as DNA from bacteria grown fully in "light" medium, and one population would run at a position halfway between fully "heavy" and fully "light" DNA. B. After the second round of replication, all of the DNA would run at a position intermediate between fully "heavy" DNA and fully "light" DNA, but closer to the "light" DNA than seen after the first round of replication (i.e., 3/4 "light"). O C. After the second round of replication, all of the DNA would run at a position halfway between fully "heavy" DNA and fully "light" DNA. D. After the second round of replication, 25% of the DNA would run at a position similar to fully "heavy" DNA and 75% would run at a position similar to fully "light" DNA.
Human Anatomy & Physiology (11th Edition)
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ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
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Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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Transcribed Image Text:**Figure 6-7: Meselson-Stahl Experiment**
This figure illustrates the results and interpretations from the Meselson-Stahl experiment, which is crucial for understanding DNA replication.
**Condition A:**
- **Bacteria Grown in Light Medium**
- **Result:** When subjected to centrifugal force, the DNA forms a band towards the top of the tube.
- **Interpretation:** These are light DNA molecules. The DNA was synthesized with lighter nitrogen isotopes.
**Condition B:**
- **Bacteria Grown in Heavy Medium**
- **Result:** The DNA forms a band towards the bottom of the tube under centrifugal force.
- **Interpretation:** These are heavy DNA molecules, indicating synthesis with heavier nitrogen isotopes.
**Condition C:**
- **Bacteria Grown an Additional 1 Hour in Light Medium**
- **Transfer Step:** Bacterial DNA initially grown in heavy medium is transferred to light medium and grown for an additional hour.
- **Result:** After centrifugation, the DNA forms a band in the middle of the tube.
- **Interpretation:** These are DNA molecules of intermediate weight. This indicates that the DNA consists of one strand of heavy DNA and one strand of light DNA, supporting the semiconservative model of DNA replication.
Transcribed Image Text:Transcription:
---
transferred to "14N-containing "light" medium for one cell division. The results of this experiment ruled out the "conservative" model of DNA replication, but to distinguish between the "semiconservative" model and the "dispersive" model, the DNA had to be heated to separate the two strands.
You are repeating the experiment, but are unable to heat the DNA to perform the second part. However, you realize that allowing the bacteria to undergo a second round of division would also distinguish between the different models. Which of the following would you expect to see as a result after the second division in "light" medium?
Choose one:
- A. After the second division, one population of DNA would run at the same position as DNA from bacteria grown fully in "light" medium, and one population would run at a position halfway between fully "heavy" and fully "light" DNA.
- B. After the second round of replication, all of the DNA would run at a position intermediate between fully "heavy" DNA and fully "light" DNA, but closer to the "light" DNA than seen after the first round of replication (i.e., 3/4 "light").
- C. After the second round of replication, all of the DNA would run at a position halfway between fully "heavy" DNA and fully "light" DNA.
- D. After the second round of replication, 25% of the DNA would run at a position similar to fully "heavy" DNA, and 75% would run at a position similar to fully "light" DNA.
---
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