transferred to *"*N-containing "light" medium for one cell division. The results of this experiment ruled out the "conservative" model of DNA replication, but to distinguish between the "semiconservative" model and the "dispersive" model, the DNA had to be heated to separate the two strands. You are repeating the experiment, but are unable to heat the DNA to perform the second part. However, you realize that allowing the bacteria to undergo a second round of division would also distinguish between the different models. Which of the following would you expect to see as a result after the second division in "light" medium? Choose one: O A. After the second division, one population of DNA would run at the same position as DNA from bacteria grown fully in "light" medium, and one population would run at a position halfway between fully "heavy" and fully "light" DNA. B. After the second round of replication, all of the DNA would run at a position intermediate between fully "heavy" DNA and fully "light" DNA, but closer to the "light" DNA than seen after the first round of replication (i.e., 3/4 "light"). O C. After the second round of replication, all of the DNA would run at a position halfway between fully "heavy" DNA and fully "light" DNA. D. After the second round of replication, 25% of the DNA would run at a position similar to fully "heavy" DNA and 75% would run at a position similar to fully "light" DNA.

5'-GCGGTACGTTACGGCTTTACTGACCTGCAGGC-3' A. Convert this to a double strand DNA molecule by writing the complementary sequence. Be sure to correctly label the ends of the double stranded molecule B. Pick one of the two strands and write the sequence of an RNA molecule that could be transcribed from it. Again, you must correctly label the ends of the molecule.

Imagine a bacterial cell with a mutation that renders helicase completely nonfunctional (note that this would be a lethal mutation). What, precisely, would go wrong with replication in this cell? Please describe what goes wrong, as well as all downstream effects.

Please help me, this is from practice, double check your answers, previous tutors got it wrong