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To make 200 ml 0.4XSSC (Saline and Sodium Citrate) solution for FISH post-washing, how many ml 20X SSC solution is needed?
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- To make 100 ml 0.3% NP-40 solution for FISH post washing, how many ul 100% NP-40 solution is needed?Given a BSA stock solution of 4mg/mL how would you prepare a 5 mL sample with a final concentration of 1 mg/mL?A pharmacist prepares 80 mL of an isotonic solution of 1.1 %w/v Lidocaine HCl (E-value 0.2). To prepare the solution, he dissolves Lidocaine HCl in purified water to make an isotonic solution. Then he dilutes the Lidocaine HCl solution with 0.9%w/v sterile sodium chloride to complete the formulation. How many milliliters of purified water is needed to make the formulation by this method?
- How do you prepare a 100mL of 0.1 M phosphate buffer? From the anterior buffer, how do you make 100mL of 0.05 M? PLEASE GIVE THE MEASUREMENTSYou receive an order for 24 mEq calcium gluconate in 50 mL normal saline. What volume, in mL, of calcium gluconate 10% stock solution is needed to provide the number of mEq ordered? Cation Aluminum Ammonium Calcium Ferric Ferrous Lithium Magnesium Potassium Sodium Formula Valence Formula weight A13+ 3 1 2 3 2 1 2 1 1 NH₂+ Ca²+ Fe³+ Fe2+ Li+ Mg2+ K+ Na+ 27 18 40 56 56 7 24 39 23 Anion Acetate Bicarbonate Carbonate Chloride Citrate Gluconate Hydroxide Lactate Phosphate, monobasic Phosphate dibasic Sulfate Formula Valence C₂H30₂ 1 HCO3 1 CO3²- 2 CI- 1 3 1 1 1 1 C6H5O7³- C6H₁107 OH- C6H5O3 H₂PO4 HPO4²- SO₂²- 2 2 Formula weight 59 61 60 35.5 189 195 17 89 97 96 96You need to make 20 mL of a 1/100 (10-2) dilution of a crystal violet solution? How much diluting fluid will be required? Note: Your answer should be in numerical format to the nearest 0.1 mL.
- How many mL of 10 x stock solution would you need to make 118 mL of 1x PBS? Report your answer to 1 decimal place.The original concentration in a sample of kombucha is 2.79 x 10^6 CFU/ml. Which dilution would yield a countable plate? How would you make this? Show your calculations. Confused as to what's implied by "which dilution"? does it mean, how many times?A 5% dextrose in 1/2 normal saline (D5 1/2 NS) solution is commonly administered to patients needing post-operative IV fluids. How could you prepare 500 mL of this solution? Dextrose is 5% (m/v) and the NaCl is 0.45% (m/v).
- 50 mL of 5mM NaCl solution from 1 M NaCl solutionIf a pharmacist needs to prepare a bacterial suspension with a concentration of 2.4x10^2 cfu using 1 mL of 2.4 x 10^4 bacterial suspension, what size of volumetric flask will she/he be needing?3 mL of a 45 mM stock solution of a substrate is added to 8 mL of water. Calculate the following values. The substrate molecular weight is 125 g/mol. Calculate the following values: Substrate Volume in mL Dilution Factor Substrate number of moles Substrate concentration for the diluted solution in mM Substrate concentration for the diluted solution in mole/L Substrate concentration for the diluted solution in mg/mL