Anonymous posted 8 Microbial Growth DQ Question: Why does turbidity lose reliability at high cell concentrations when the culture reaches the stationary phase?

Please answer fast Dilution Problem. A culture of Staphylococcus is diluted as follows: (1) 20mL are added to 80mL of water. (2) 10uL from (1) are then added to 9.99mL of water. (3) A 10-2 dilution is made from tube # (2). (4) 100uL from (3) are plated for a pour plate and incubated.   Growth Problem.A culture with approximately 2x103 cells/mL were incubated. After 3 hours, the number of cells had increased to 3x105. a) How long was the generation time in minutes?b) How many generations have occurred

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wwwwww The total number of cells in a culture is counted using dye trypan blue and is found to be 3.8 x 106 cells/mL. The culture is diluted 1:22 and then 200μl seeded per well into a 96 well plate. What is the final cell density per well? ssibility: Good to go MacBook Air

Explain the advantages and disadvantages of Differential Centrifugation, main differences btw. Differential and Gradient Density Centrifugation and why the differential Centrifugation is a process of successive centrifugation?

One step is called the "lag" phase. Does this phase always occur? Explain.

Table 3-Determination Tube # Potato Expt. Temp. 2c 3c 4c 5c extract (mL) 2 2 2 2 room temp 20 °C 40 °C 75 °C of the optimum temperature of catechol oxidase enzyme. 1st Absorbance 0 min. at Expt. Temp. Boiling 100 °C dH₂O Catechol (mL) (mL) 0 0 0 0 13 13 13 13 Start Time: 4:20 Absorbance: 0.072 Start Time: 4:25 Absorbance: 0.114 Start Time: Absorbance: 25 0.132 Start Time: 4:27 Absorbance: 0.097 2nd Absorbance after 10 min. in Expt Temp. Time for reading: 4:30 Absorbance: 0.128 Time for reading: 4:33 Absorbance: 0.036 0-137 Time for reading: 4:35 Absorbance: Subtract 1st from 2nd absorbance 0.193 Time for reading: 4:37 Absorbance: 0-126 0.023 0.061 0.029 Based on the data from Table 2 answer these questions: Q10) What is the enzyme's optimum temperature? Answer: Q11) Use the difference between 2nd and 1st absorbance to support your conclusion for the optimum temperature 3rd Absorbance after 10 more min. at Room Temp. Time for reading: 4:40 Absorbance: 0.159 Time for reading: 4:43…