The original solution was diluted by a factor of three, and then the absorbance of the diluted cereal sample solution was measured and recorded as (2.0x10^-1). Use the following trendline and dilution factor to determine the concentration of iron, in unit of M, in the original cereal sample solution. The trendline (linear regression) as displayed on the plot is y = 11898x - 0.0112.
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The original solution was diluted by a factor of three, and then the absorbance of the diluted cereal sample solution was measured and recorded as (2.0x10^-1). Use the following trendline and dilution factor to determine the concentration of iron, in unit of M, in the original cereal sample solution. The trendline (linear regression) as displayed on the plot is y = 11898x - 0.0112.
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- The spectroscopic data in the table is generated with five solutions of known concentration. Concentration (M) 0.0133 m= 0.0266 0.0532 0.106 0.213 Absorbance 0.1271 What is the intercept of the linear regression line? 0.08531 0.5388 1.069 Use a spreadsheet program, such as Microsoft Excel, to graph the data points and determine the equation of the best-fit line. 1.954 What is the slope of the linear regression line formed by these points? M-1After determining the absorbance of several standards of known concentration, the trend line for a calibration curve plotting absorbance (y-axis) against concentration (uM, x-axis) is determined to be y=4.244x+0.0000. The absorbance of a solution of unknown concentration is determined to be 0.604. Calculate the concentration of the unknown solution in microM. Give answer to 3 decimal placesWhat are the differences between systematic and random errors and how do they effect accuracy and precision? In what circumstances would you use standard addition (versus a normal calibration curve) to determine the amount of an analyte in a sample? A urine sample, containing analyte Z is analysed by the standard addition method where 5 mL of the original sample was mixed with increasing amounts of a Z standard and each solution diluted to a volume of 50 mL prior to analysis. A plot of the final concentration of the standard in each of the 50 mL samples (x axis) versus The measured signal from the analysis of each 50 mL sample (on y axis) produced a straight line with the general equation: y = 44.72x + 4.06 what was the final concentration of Z in the 50 mL standard addition sample? what was the initial concentration of Z in the original urine sample?
- To determine the molar concentration of a metal ion in a solution of unknown concentration, a student fırst made five standard solutions that contain the metal ion of interest and measured the absorbance of each solution in a spectrophotometer at its Amax- A calibration curve was obtained that had an equation of y = 5.747 x + 0.013 Next, the student pipetted 15.0 mL of the initial solution of unknown concentration into a 100.0 mL volumetric flask, and filled the flask with deionized water to the line. The absorbance of this final diluted solution was found to be A = 0.226 at Amax. The color of the original and diluted solution was blue. What is the molarity of the original solution, as well as an approximate Amax for this metal ion? 2max = 599 nm and concentration is 0.247 M Amax 457 nm and concentration is 0.247 M %3D 2 max = 457 nm and concentration is 0.0371 M 1 max 599 nm and concentration is 0.0371 M Amax = 599 nm and concentration is 0.00557 MThe protein concentration of a protein isolate was determined using the Bradford assay. Five dilution of bovine serum albumin (BSA) were prepared from a 5.00 mg/mL BSA stock solution. To these dilutions, 100 µl of Bradford reagent were added. After 5 minutes, the absorbance was taken at 595 nm. Standard # BSA, in mg/mL Volume of Bradford reagent, A595 in mL 1 5.0 0.000 1.00 5.0 0.134 3 2.00 5.0 0.265 4 3.00 5.0 0.388 4.00 5.0 0.497 The supernatant dilution was prepared by mixing 49 µL of the protein isolate with 16 µL water. The absorbance was taken 5 minutes after addition of 100 mL Bradford reagent. Calculate the protein concentration (in mg/mL) of the original isolate if the absorbance at 595 nm was 0.413. Note: Final answer format must be x.xxx (three decimal places). Round off only in the final answer. Do not round off in the middle of calculation.After determining the absorbance of several standards of known concentration, the trendline for a calibration curve (AKA a standard curve) plotting absorbance (y-axis) against concentration (M, x-axis) is determined to be y = 4.943x + -0.0001 The absorbance of a solution of unknown concentration is determined to be 0.48. Calculate the concentration of the unknown solution in M. Give your answer to three decimal places.
- The absorbance of an unknown dye solution is measured to be 1.06 by a spectrometer. The calibration plot of the spectrometer is provided below. What is the molar concentration of the unknown sample (in mole L)? Absorbance vs. Molarity 18 y3 67024x-0.0343 16 14 12 1 0.8 0.6 0.4 0.2 01 0.2 0.000005 00001 0000015 Concentration of solutions, mol/L 0.00002 0.000025 000003 0 163e-5 O 2.35e-6 0 163 0 235 AbsorbanceWhat are the advantages and disadvantages of volumetric and gravimetric analysis?A student was given a stock aluminum(III) solution with a concentration of 5.000 parts per million (ppm). (ppm are defined as mg/L for dilute aqueous solutions.) The student prepared five 100 mL standard solutions and an unknown as described in the procedure section of the experiment. The absorbance of each solution was read at 565 nm, using the blank to set zero absorbance. The results are tabulated below. Solution Volume of Al(III) stock solution used (mL) Absorbance 1 8.00 0.701 2 6.00 0.548 3 4.00 0.378 4 2.00 0.208 5 1.00 0.123 Unknown --- 0.534 3. What is your best estimate of the Al(III) concentration of the unknown sample in the cuvet based upon where its absorbance falls on the standard curve?
- The absorbance of an unknown dye solution is measured to be 0.47 by a spectrometer. The linear equation of the spectrometer's calibration plot is y= 2156.5 x +0.0213, where y represents absorbance and x represents concentrations of dye. What is the molar concentration of the unknown sample (in mole L)? O2.08e4 O 2.08e-4 0 125e3 0 125e-3The glucose content of samples can be determined using Nelson’s test. The equation of the line for the standard curve prepared for varying concentrations of glucose is y = 3.0x + 0.2. Based on this information, determine the glucose concentration (in M) of a mango preserve having an absorbance reading of 0.891.A water sample was analyzed for Fe content using the iron-phenanthroline method. The following data were obtained from the analysis: Determine the value of the slope (m), y-intercept (b), and the coefficient of determination (r2)