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What is the difference between isocratic and gradient elution?
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- Why is gradient elution usedWhat is the purpose of a calibration curve in analytical chemistry?4.Consider the peaks for pentafluorobenzene and benzene in the gas chromatogram shown here. The elution time for unretained solute is 1.06 min. The open tubular column is 30.0 m in length and 0.530 mm in diameter, with a layer of stationary phase 3.0 μm thick on the inner wall. a) Measruing the width, w, at the baseline on the chromatogram, find the number of plates for these two compounds b) Use your answer to (a) to find the resolution between the two peaks c) Using the number of plates N=sqrtN1*N2 with the values from (a) calcuate what the resolution should be and compare your answer with the measured resolution in b
- 4. A new method for the determination of mercury in fish was tested against a N.I.S.T. standard to determine if there was any bias (determinate error) in the new technique. The N.I.S.T. standard fish sample was certified to contain 13.55 ppm Hg. The results of six analyses by the new method gave a mean value of 14.15 ppm and s.d. = 0.42 ppm. Does the new method give accurate results at the 95% confidence level?Chromatography involves the separation of two or more compounds/ions caused by their molecular interactions with two phases. True FalseThe researcher extracts a sample of well water and prepares it for TXRF analysis with a 10.00 mg/L V internal standard, like the calibration standards. The result produces SCa = 69297.81 and SV = 13481.2.Determine the concentration of Ca.
- The following values were obtained for the calibration of a 10-mL pipet: 10.15, 9.95, and 10.00 mL. Calculate the mean value and the average deviation from the meanChemistry Fraction no. Elution Vol. (mL) Abs (λ = 410) Fraction no. Elution Vol. (mL) Abs (λ = 410) 1 (PB pH 6.0) 1 0.0050 11 11 0.0041 2 2 0.0032 12 12 0.0060 3 3 0.0040 13 13 0.0064 4 4 0.0064 14 14 0.0063 5 5 0.0067 15 15 0.1109 6 6 0.0067 16 16 2.8902 7 7 0.0050 17 17 0.8500 8 (PB pH 8.0) 8 0.0049 18 18 0.2019 9 9 0.0052 19 19 0.0378 10 10 0.0050 20 20 0.0073 Use the data above to plot an elution profile, in which the absorbance is on Y-axis, and elution volume (volume = fraction number) is on X-axis. The elution profile must indicate at (1) which point the pH change occurred, (2) the fraction(s) which contain the highest myoglobin content, and (3) the maximum absorbance detected.The ages of a group of 122 randomly selected adult females have a standard deviation of 18.5 years Assume that the ages of female statistics students have less variation than ages of females in the general population, so let o = 18 5 years for the sample size caloulation How many female statistics student ages must be obtained in order to estimate the mean age of all female statistics students? Assume that we want 95% confidence that the sample mean is within one-half year of the population mean. Does it seem reasonable to assume that the ages of female statistics students have less variation than ages of females in the general population? The required sample size is (Round up to the nearest whole number as needed)