The spectrophotometer is set at 600 nm. The absorbance of each cuvette is read. The data is shown below: Cuvette 1 is the clank Cuvette # ml albumin Absorbance 1 2 0.2 .063 3 0.4 163 4. 0.6 .187 0.8 .253 1.0 .289 Using this data, plot an Excel graph showing absorbance (y-axis) vs ml albumin (x-axis). Find the correlation coefficient (r). R2 > 0.99 excellent data; r2 0.98-0.95 good data r2 0.94-0.90 = fair data. How would you describe this data?-

* Question Completion Status: QUESTION 6 Most analytical machines used in science can either measure absorbance of light, like the spectrophotometers used in BioZ 151 and 152 labs, or can measure change in electrical conductance. In order to convert things like absorbance values into concentration data, a linear regression is used. In order to determine nitrite concentration in water samples, nitrite is reacted with several chemicals to produce a purple color. The absorbance of the solution is measured from known amounts of nitrite to produce a standard curve to analyze samples. Use the data in the provided table, or from the linked spreadsheet to make a graph and perform a linear regression using treadline. mg/L NO, Absorbance 0.1 0.183 0.3 0.5 0.7 0.542 0.914 1.298 1.61 In order for this to work, samples must be diluted prior to measurement standard curve. If a sample underwent a 0.02 dilution, and produce an absorbance value of 0.903, what is the resultant concentration of NO2 in mg…

The concentration of methylene blue in the sample is estimated through (select the correct)   options:   refractive index measurement (refractometry).   turbidity measurement (turbidimetry).   absorbance measurement (spectrophotometry).   titration with 2% HCl solution.

A student weighed out 0.150 g of protein powder and dissolved it in 100 mL of water (Solution 1). The student then diluted this solution by transferring 1 mL into a 25 mL flask and diluting with water (Solution 2). Finally, 1 mL of that solution was transferred to a test tube and combined with 4 mL Bradford reagent. The absorbance of the solution in the test tube was 0.187. Assuming that the best fit linear line of the standard curve was y = 0.04144 x + 0.01521 (μ g mL), calculate the percent protein by mass in the original protein powder.

A solution containing a mixture of the compounds X and Y had an absorbance of 0.814 at 443 nm and an absorbance of 0.699 at 520 nm when measured with a 1.00 cm cell. The molar absorptivities (e) of X and Y at each wavelength are shown in the table. [X] = Wavelength (2, nm) [Y] = 443 Incorrect 520 2.13 x10-5 1.23 x10-4 Incorrect Molar Absorptivity (c. M-¹cm-¹) X What are the concentrations of X and Y in this mixture? 16180 3936 Y 3817 6333 4 M M

How do you convert absorbance to µmoles?

Sel Absorbance S Verify your molar absorptivity value. Did you report your data to the correct number of significant figures? Analytical wavelength Analytical wavelength for blue dye (nm) Calibration curve Solution 1 2 3 4 5 Dye concentration (μM) 15.20 7.51 Unknown solution 3.74 1.89 1.01 Molar absorptivity (μM ¹cm-¹) Absorbance Concentration of unknown blue dye (µM) 629 Absorbance 0.756 0.432 0.259 0.175 0.144 0.083 How to calculate concentration from absorbance S A d tra 01

After creating her standard curve for absorption versus the concentration (M) of FD&C Red 40, a student found that her best fit linear line for FD&C Red 40 was y = 2,962x + 0.005. Her Kool-Aid sample had an absorbance of 0.685. If 0.543 grams of Kool-Aid powder was used to prepare an 8-fl oz cup of her assigned flavor, what is the percent by mass of FD&C Red 40 in her 8-fl oz cup?

After determining the absorbance of several standards of known concentration, the trendline for a calibration curve (AKA a standard curve) plotting absorbance (y-axis) against concentration (M, x-axis) is determined to be y = 4.586x + -0.0010 %3D The absorbance of a solution of unknown concentration is determined to be 0.68. Calculate the concentration of the unknown solution in M. Give your answer to thrėe decimal places.

How would I do 2? the concentration iron is 1.85mg/vitamin tablet, and the absorbance at 508nm is 0.37