A standard of solution was put through appropriate dilutions to give the concentrations of glucose (mM) shown in the accompanying table. The absorbances in the table (1.00-cm cells) were recorded at 490 m table. Use the method of least squares to find an equation relating absorbance and the concentration of glucose.

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Spectroanalytical Methods

Spectroanalytical methods measure the wavelength and intensity of radiations that are either emitted or absorbed by a molecular or atomic species. These methods are useful for evaluating the electronic or atomic arrangement of the molecules of a given analyte.