The result of a TLC experiment showed that a compound has an Rf value of 0.25 on a silica gel plate using 10% ethyl acetate in hexane as the mobile phase. Indicate if the Rf values of the compound would be higher or lower in each of the following solvents. Breifly explain your reasoning for full credit: (a) 100% hexane (b) 100% Toluene (c) 50:50 of hexane ethyl acetate (d ) 95:5 of ethyl acetate: methanol

The result of a TLC experiment showed that a compound has an Rf value of 0.25 on a silica gel plate using 10% ethyl acetate in hexane as the mobile phase. Indicate if the Rf values of the compound would be higher or lower in each of the following solvents. Breifly explain your reasoning for full credit: (a) 100% hexane (b) 100% Toluene (c) 50:50 of hexane ethyl acetate (d ) 95:5 of ethyl acetate: methanol

EBK A SMALL SCALE APPROACH TO ORGANIC L

4th Edition

ISBN:9781305446021

Author:Lampman

Publisher:Lampman

Chapter89: Thin-layer Chromatography

Section: Chapter Questions

Problem 7P

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From the study with UV-Vis spectroscopy, four samples of solutions with different known concentrations of atrazine were analyzed, using a 1 cm optical path cuvette at 220 nm. The analytical curve (calibration curve) with absorbance values (y-axis) per atrazine concentration (x-axis) provided a straight line with the following parameters: linear coefficient = 0 and slope = 760. assumptions of the Lambert-Beer Law, evaluate the following statements: I. The molar absorptivity coefficient (E), under the described experimental conditions, cannot be determined. II. A sample of a solution containing atrazine with an absorbance of 0.2 will show a concentration of 2.6x10-4 mol/L. III. Atrazine concentrations could be determined due to absorption taking place in the visible region. IV. The change in absorbance as a function of the analyte concentration is directly related to the absorptivity coefficient of atrazine and the length of the optical path of the cuvette. It is correct only what is…
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