Indicate the order of elution in which the following compounds would be eluted from an HPLC column containing a reversed-phase packing: (a) benzene, diethyl ether, n-hexane (b) propylene, hexane, benzene, dichloro-benzene
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Indicate the order of elution in which the following compounds would be eluted from an HPLC column containing a reversed-phase packing:
(a) benzene, diethyl ether, n-hexane
(b) propylene, hexane, benzene, dichloro-benzene
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- TLC was used to compare the solubility of two compounds A and B in a solvent system (acetic acid, methanol, H2O). The distances that solvent has moved in the silica gel TLC was 12 cm. and for the compounds were 2 and 8 cm respectively. (a) Calculate the Rf values and which compound is more soluble and which one is more polar. (b) What are the factors affecting the Rf valueWhat is the order in which the following compounds would he eluted from an HPLC column containing a reversed -phase packing?(a) benzene, diethyl ether, n-hexane(b) acetone, dichloroethane, acetamide(h) Describe a chromatographic technique that could be suitable for studying the molecular weight distribution of poly(phenylenevinylene) derivatives that are soluble in organic solvents. Explain the physical basis on which separation could occur and suggest how the polymers could be detected after elution.
- Predict the order of elution for the silica gel adsorption TLC separation of the mixture containing aspirin, acetaminophen and caffeine using methylene chloride solvent. Explain.Micellar electrokinetic capillary chromatography was conducted at pH 10 with anionic micelles and the anode on the sample side. (i) Is aminobenzene (C6H5NH2) a cation, an anion, or a neutral molecule in this experiment? (ii) Explain how to decide which compound, aminobenzene or anthracene, is more soluble in the micelles.In color chromatography of plant pigments, what complications would a dried-out column (solvent level is below the top of the silica) introduce to the elution and isolation of pigments?
- Explain why TLC cannot be used for some volatile low molecular weight substances such as toluene.Predict the order of elution of the following compounds from a GC column packed with polyethylene glycol:hexane, heptane (b.p. 98oC), octane (b.p. 126oC), benzene (b.p. 80oC), butanol (b.p. 117oC), 2-pentanone (b.p.102oC)A student developed a chromatogram and found that the eluent from travelled 94 mm and the Zn^2+ cation travelled 24 mm. In the development of a chromatogram of a mixture of cations, the eluent front travelled 87 mm. if Zn^2+ is a cation of the unknown mixture, where will its band appear in the chromatogram?
- Column chromatography is also very useful for separating mixtures of organic compounds that are not coloured. Briefly explain how you could use column chromatography for the separation of a colourless mixture, and how you could identify your separated compounds.1. From spinach, the chromatographic analysis will yield two major substances: (a) B-carotene (C40H56), which will separate from the spinach mixture to form a yellow band, and (b) chlorophyll A (CSSH72MGN4OS), which will separate from the mixture to form a green band. i. Describe the characteristics and draw structures of these molecules. ii. From the spectrophotometric analysis, what wavelength (Amax) would you expect ß-carotene (C40H56) to absorb? What wavelength would chlorophyll A (CssH72MgN4O5) absorb?Diethylamine (C4H₁1N) and n-butylamine (C4H₁1N) are colorless liquid amine compounds, suggest chromatographic conditions (stationary and mobile phases, pH of the sample) in order to separate these compounds using IEC. Predict the retention.