The protein gamma globulin has an isoelectric point of 6.60. Suppose you did a pH titration of a solution containing gamma globulin. At what pH will the protein aggregate?
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The protein gamma globulin has an isoelectric point of 6.60. Suppose you did a pH titration of a solution containing gamma globulin. At what pH will the protein aggregate?
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- Diethylaminoethyl cellulose is a positively charged resin used in ion-exchange chromatography with a pKa is about 11. A negatively charged protein with an isoelectric point of 5.0 is applied to the column in a buffer at pH 7 and containing 0.1 M NaCl. Which of the following conditions is most likely to weaken the interaction between the protein and the resin? A Raising the pH to 8 and decreasing the NaCl to 0.05 M B Raising the pH to 8 and increasing the NaCl to 0.2 M C Lowering the pH to 6 and decreasing the NaCl to 0.05 M D Lowering the pH to 6 and increasing the NaCl to 0.2 MA protein has a sedimentation coefficient of 2.6S, when ultracentrifuged in a 0.1 M NaCl solution; the same protein has a sedimentation coefficient of 4.6S, when centrifuged in a 1.0 M NaCl solution. This change in the sedimentation rate at 1.0 M NaCl, most likely occurs because the protein. (one word = verb).A solution containing 3.58 x 1023 molecules/m3 of protein in water is separated from pure water by a membrane 3.20 μm thick. The diffusion coefficient of the protein through the membrane is 7.15 x 10-18 m2/s. On average, how many molecules cross 0.0240 μm2 of this membrane each second? [Answer as a positive number with 3 sig digits, but do not enter units with your answer]