The piRNA, miRNA and RNAi pathways are all similar and may have evolved from some early function of small RNAs in an ancestral cell. The piRNA pathway seems a bit unique in several ways however. Name two distinct features that are unique to the piRNA pathway when compared to the miRNA and the RNAi pathwa

Transfer RNA in eukaryotic cells is synthesized by which of the following enzymes (sensitive to high concentrations of the fungal poison a-amanitin, but not to low concentrations)? RNA polymerase I DNA polymerase I RNA polymerase II DNA polymerase II RNA polymerase III Which of the following processes of genetic information flow can occur under laboratory conditions, but has never been observed to occur under natural conditions (either in living cells or in viruses)? transcription of RNA from a DNA template (using DNA-dependent-RNA-polymerase) self-replication of RNA from an RNA template (using RNA-dependent-RNA-polymerase) direct-translation of protein from a DNA template (using special ribosomes) self-replication of DNA from a DNA template (using DNA-dependent-DNA-polymerase) translation of protein from an RNA template (using ordinary ribosomes)

A doctoral student in biology, Esther Mensah, is reviewing primary literature over RNAi interference as part of her literary research for her proposal on decreasing the amount of abnormal white blood cell growth associated with acute myeloid leukemia (AML) using a murine (mouse) model. She is being provided with 10 AML mice courtesy of her mentor. Can you help her out with her proposal?   1)  Given her knowledge of RNAi, what type of RNA will Esther use to target the murine AML gene?   a piRNA. b circRNA. c lncRNA. d siRNA.   2)  In order for Esther’s RNAi to work, the small RNA pieces have to interact with another  molecule. What molecule does she hope it will bind to? a DUH! It will bind to the DNA strands of the AML gene and knock it out! b It will bind to tRNA, which will block attachment of the amino acids needed to make the AML protein. c It will bind to circRNA, which will increase its longevity. d It will bind to the…

A double-stranded RNA has been generated by release/splicing out of an intron within the nucleus...outline the pathway taken to generate microRNA and its  action on the shutdown of protein biosynthesis

TFIID TFIIA TFIIH TBP TFIIB TFIIF TFIIE

The siRNA and miRNA pathways share many similarities and also have some differences. Which of the following is true about these two noncoding RNA classes in regulating gene expression? O Both of them utilize the internally generated RNAS as sources to produce the short regulatory RNAS O Both of them need Dicer to process the long RNAS into short double-strnaded RNAS Both of them required perfect match between the short noncoding RNAS to the MRNAS Both of them end up with complete degradation of the target mRNAs