The picture shows bacteria (Escherichia coli) under a microscope Do you bacterial growth or no ( negative and positive) And explain? (Is there growth or not?)
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- Imagine that you are Gram staining a mixed culture of both gram-positive and gram-negative bacteria. If you omitted the iodine (mordant) step in the procedure, what colors would the bacteria on your slide appear to be? ► The gram-positive bacteria would be purple, and the gram-negative would be pink All of the bacteria would look purple All of the bacteria would look pink The bacteria would not be stained at all (clear)The image is showing Bacillus subtillis bacteria under 400x magnification, the same magnification used on the plant and animal photos. Why are the bacterial cells so much harder to see in this microscope image?Suppose you are staining three identical smears of the same bacterial culture with three different basic simple stains: carbol fuschin, crystal violet and methylene blue. You observe each stained preparation under oil immersion at 100X. which of the following bacterial characteristics would you expect to be different between each of the stains as you view them under the microscope. Bacterial arrangement Bacterial shape Size of cells The color of the bacteria
- 39 You perform a Gram stain and when you look down the microscope see the image shown. You can conclude about (B): Select one alternative: O It is a Gram positive rod O The cell wall has a thick layer of peptidoglycan O It is a Gram positive bacteria The cell wall has a thin layer of peptidoglycan O It is a Gram negative cocci(Two part question I have separated for clarity) Which stains are used to differentiate between the cell walls of bacteria? What CAN”T the Gram stain tell you about a bacteria?Before viewing a specimen of pigmented bacteria on a slide under the light microscope, which of the following usually needs to happen (and why)? Staining (to increase magnification). Heat, radiation or antimicrobial chemical treatment (to kill the bacteria for safe observation). Add immersion oil (to increase resolution). Add a cover slip (to reduce contamination). The viewing chamber needs to be flushed of air (to create a vacuum).
- Using an appropriate lens and oil immersion, what would be a microscope's maximum resolution (in micrometres) that could use a wavelength of 350 nm (assuming that such a device exists)? What would be the surface-to-volume ratio for the coccus-shaped bacterium twice the size of the maximum resolution you calculated? Why is the surface-to-volume ratio an important biological factor. Explain. Show all of your calculations.This is a compound light microscope O dark-field illumination microscope O scanning electron microscope transmission electron microscope 71°F Sunny 立Examine the image below. The bacteria in this image have been treated with gram straining procedure. Indicate the following information: A. Shape....... gram stain( +) , Gram (-) or both B. Stained color under microscope C. PG Wall thick, thin or both
- Describe in detail all the steps needed to determine whether an Unknown bacteria has a Gram-positive or Gram-negative cell wall using the Gram stain. (You can describe the method presented in the Exercise 3-6 Gram stain videos or in the Lab Manual.) 1. Start by describing the method of transferring the bacteria onto the microscope slide, then describe heat-fixing the emulsion of the Unknown bacteria. (Do not describe all the steps needed to perform aseptic technique. Just say “Bacteria was added aseptically.”) 2. Describe the Gram stain in detail. (This description should include all the stains, how long the stain should set on slide, and Genus and species names of bacteria you are using as CONTROLS.)Cellular stains are commonly used in bacteriology. Which of the following is NOT a reason to stain a bacterial preparation. Cellular stains are commonly used in bacteriology. Which of the following is NOT a reason to stain a bacterial preparation. Determine metabolic properties of a cell. To determine the shape of the bacteria in the culture. To determine what type of cell wall the bacteria has (e.g. gram positive or Gram negative) Identify specific features of a cell (spores, flagella etc)a. What can you observe in viewing your stained bacterial slide under the microscope if you fixed a lot of bacterial colonies in your slide during smear preparation? b. What can you observe in viewing your stained bacterial slide under the microscope if you heat fixed your slide in a much longer exposure to heat?