The Light conditions were random, including No light, Red light, and Green light. Please note that chlorophyll preferentially absorbs the light with wavelength of 660 nm (red) when compared to that of 520 nm (green). Your mission is to determine which Tube came from which experimental light condition. Tube: Tube A Tube B Tube C Abs: 0.260 2.22 1.72 Table 1: In the table below, copy the Abs readings you recorded above and then write the light condition (described in first paragraph) which you think the Tube was exposed to during the experiment. Tube Abs. Light Condition A 0.260 B 2.22 C 1.72 Explain the reasoning behind the assignments you made in above table.
At your work station, you have a spectrophotometer set at 600 nm to measure DCIP absorbance and four (4) test tubes containing different solutions. One of the test tubes is labelled “0” to blank/zero the spectrophotometer. The other three (3) test tubes contain a reaction mixture from a Hill reaction experiment using isolated chloroplasts and DCIP as an electron acceptor. DCIP is present in the oxidized state and exhibits a blue color with an absorbance at 600 nm. Upon receiving the electrons, DCIP is reduced to form DCIP-H2 that is colorless. These three (3) tubes, labelled “A”, “B”, and “C” were exposed to different light conditions during the Hill reaction.
The Light conditions were random, including No light, Red light, and Green light. Please note that chlorophyll preferentially absorbs the light with wavelength of 660 nm (red) when compared to that of 520 nm (green). Your mission is to determine which Tube came from which experimental light condition.
|
Tube: |
Tube A |
Tube B |
Tube C |
|
Abs: |
0.260 |
2.22 |
1.72 |
Table 1:
In the table below, copy the Abs readings you recorded above and then write the light condition (described in first paragraph) which you think the Tube was exposed to during the experiment.
|
Tube |
Abs. |
Light Condition |
|
A |
0.260 |
|
|
B |
2.22 |
|
|
C |
1.72 |
|
Explain the reasoning behind the assignments you made in above table.
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