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- If the diameter of the low power field is 1.5 mm, an object that occupies approximately a 1/3 of that field has an estimated diameter of what mm?Both spotlights SPOTLIGHT#1 and SPOTLIGHT#2 are place at 20 meters from the stage. You want to produce a circle of light of 1 meter diameter with the SPOTLIGHT#1and a circle of light of 2 meters diameter with the SPOTLIGHT#2if the absorbance value measured in a spectrophotometer is 0.5, how much of the original incident light is being measured by the detector?
- A solution containing 2g/liter of a light-absorbance substance in 1 cm cuvette transmits 75% of incident light of a certain wavelength. Calculate the transmission of a solution containing 4g / literAssume that you have an “X” solution that you do not know its concentration. But you have other X solutions with known concentrations and you know X solution absorbs maximum light at 575 nm. To calculate the unknown solution concentration, you have done some spectrophotometric measurements and obtained data given below. Prepare a standard curve by using the absorbance of known concentrations (Hint: you can learn how to prepare a standard curve by using Excell from youtube). Calculate the concentration of unknown.The amount of chlorophylla (M, 892) in a spinach leaf is about 20 µg/cm² of leaf. In midday sunlight, the average rate of energy reaching the leaf is 5.4 J/cm². min. Assuming an average photon energy of 270 kJ/einstein and that the leaf absorbs 50.0% of the radiation, calculate the rate of light absorption by the photons in units of einstein/cm²-min. Number rate= 1.6× 10¹4 Calculate the amount of time, in seconds, that it takes for a single chlorophyll molecule to absorb a photon. The molar mass of chlorophyll is 892 g/mol. time = Number einstein/cm². min Given that the average lifetime of an excited chlorophyll molecule in vivo is 1 ns, calculate the fraction of the chlorophyll molecules that are excited at any one time. Enter the fraction as a decimal. fraction= Number
- In absorption spectrophotometry: a. Absorbance is directly proportional to transmittance b. Percent transmittance is directly proportional to concentration c. Percent transmittance is directly proportional to the light path length d. Absorbance is directly proportional to concentrationWhat are the underlying physical principles of paper chromatography? What is spectrophotometry, the essence of it?the figure shows the result of the spectral analyzer for paper spectrophotometer without any transparent paper and with transparent yellow color paper. observe and discuss the intensity and the wavelength for both results.