THE LIGHT DEPENDEN REACTION MUST OCCUR BEFORE THE LIGHT INDEPENDENT REACTION    TRUE OR FALSE?

Suppose you were running out of substrate (PNPP) and decided to reduce the concentration used in your reactions (25 µM, 50 µM, 100 µM, 200 µM, 300 µM) by a factor of 100. How might this affect your kinetic analysis?

Differentiate non-cyclic light reaction and cyclic light reaction

Show the chemical reaction that takes place when Benedict's reagent (Cu2+) reacts with glucose (Indicate color change).

Consider the following free energy diagram for an uncatalyzed and enzyme-catalyzed reaction. Select all the statements that are true. Without enzyme With enzyme A+B Time AB Oa. The reaction is now spontaneous due to the addition of enzyme b. The rate of the enzyme catalyzed reaction is faster than the uncatalyzed reaction O C. The reaction is exergonic O d. The change in free energy for the reaction is greater in the catalyzed reaction, compared to the uncatalyzed reaction e. The enzyme stabilizes the transition state for the reaction Released Energy pes

Explain how the observation that reaction (B) runs faster than reaction (A) is related to the enzyme catalysis.

UNDER PHOTOSYNTHETIC CONDITIONS, how will the pH of the CO, indicator change? Explain why this happens. What effect will cellular respiration have on the pH? WHY IS THE "dark" cuvette wrapped in foil and then placed under a lamp? HOW DID THE results of the experiment with white light at 25 cm compare to those for blue, green, and red light at the same distance? Is this what you would expect given the composition of white light? UNDER WHICH condition(s) was the CO, indicator the most alkaline? Explain why. WHY DO MANY plants contain accessory pigments other than chlorophyll?

The aldolase reaction can proceed in reverse as an enzymatic aldol condensation. If the enzyme were not stereospecifi c, how many different products would be obtained?