The image below is an example of the results that you could expect to see from your gel. Each group (e.g., lanes labelled A) contains two samples, Bl=Blue and Br=Brown. Interpret the results below as being homozygous blue, homozygous brown, or heterozygous. A В LMW C BI Br BI Br BI Br BI Br A. В. 250 С. 200 150 D. The sample lane listed as "LMW" denotes what is known as a Low Molecular Weight marker See Figure3This is a commercially available sample set of standard DNA fragments, measured in base pairs (bp). These standards of known size are used to determine the size of your PCR products (the DNA fragments you have created by PCR) by comparing the position of your bands with the bands of known size. Based on the gels above and the image of the LMW marker below, extrapolate the size of the Blue and Brown eye colour alleles. 766 Blue eye colour allele size (bp): 500 350 Brown eye colour allele size (bp): 300 250 200 Figure 3: Low Molecular Weight DNA Marker (New England Biolabs) 150 100 75 -50 25

possibie up to 1.) 7.) The genotype of F, individuals in a tetrahybrid cross is AaBbCcDd. Assuming independent assortment of these four genes, what are the probabilities that F, offspring will have the following genotypes? (a) aabbccdd (b) AaBbCcDd (d) AaBBccDd (е) AаBBCСdd (c) AABBCCDD

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Yellow Spotted Marker 1 Marker 2 Marker 3 Marker 4 Black K8 KB KE KB I. Marker 1 Marker 2 Marker3 Marker KB k III. 1 7 8. 10 11 12 Marker 1 Marker 2 Marker 3 Marker 4 KB. -KB Gene K controls fur color and it has three alleles: B, s, and y. The pedigree shows the genotypes of the individuals in the K gene and the haplotypes for the four molecular markers in the region of the gene. a) what is the relationship between the three alleles? Use the pedigree to support your answer and refer to individuals by their positioning in the pedigree b) based on the pedigree and the haplotype at the four markers, where is gene K located? Support your answer. c) A genetic map of the 4 markers studied is shown below: Marker 1 Marker 2 Marker 3 Marker 4 JU 5 cM 6 cM 12 cM What is the probability that an offspring of a cross between l-1 and I-2 would have the haplotype below? Show your work. 3 1 1 1 1