Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN: 9780134580999
Author: Elaine N. Marieb, Katja N. Hoehn
Publisher: PEARSON
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- The operator of the lactose operon in E. Coli is located: between the promoter and the repressor gene upstream of the promoter between the promoter and the structural genes anywhere on the chromosome among the structural genesarrow_forwardThe organization and function of the lac operon in E. coli is shown in the following figures: XXI Transcription Repressor mRNA Translation RNA polymerase M M Flask ZYA XXXX Active M-repressor protein Repressor active, operon off. The repressor protein binds with the operator, preventing transcription from the operon. (a) An inducible operon Copyright © 2007 Pearson Education, Inc., publishing as Benjamin Cummings Allolactose (inducer) Carbon/Energy Source 0 Z MInactive repressor protein (a) An inducible operon Transcription Translation XXX Permease B-Galactosidase Transacetylase Operon mRNA 3 Repressor inactive, operon on. When the inducer allolactose binds to the repressor protein, the inactivated repressor can no longer block transcription. The structural genes are transcribed, ultimately resulting in the production of the enzymes needed for lactose catabolism. Copyright © 2007 Pearson Education, Inc., publishing as Benjamin Cummings Suppose you inoculate three flasks of minimal…arrow_forwardIn most experiments using the lac operon, researchers use the synthetic inducer IPTG to turn on operon expression, instead of lactose or allolactose. What do you think is the advantage of using IPTG?arrow_forward
- You are growing E. coli in a laboratory in order to study their operons. The growth media you are using contains lactose, no glucose and no tryptophan. Using your knowledge of operons and their regulation a.Which operons would be functional under these conditions? b.What repressors would be made? (NOTE: name the repressors using their gene names) c.Which repressor(s) would be made in the inactive form? d.Which repressor(s) would be made in the active form? e.Which repressor(s) under these conditions can bind the operator sequence? f.Which repressor(s)under these conditions cannot bind the operator sequence?arrow_forwardA mutation at the operator prevents the regulator protein from binding.What effect will this mutation have in the following types of operons? Q. Regulator protein is a repressor of a repressible operon.arrow_forwardIn the lac operon, the lacZ gene codes for an enzyme called ____________, which catalyze the breakdown of the sugar __________ into monosaccharides. This enzyme can also hydrolyze the sugar analog ____________, which produces blue pigments following its breakdown. Since the lac operon is not always on the bacterial cells, we add the chemical ________ which prevents the lac depressor from binding to the operators, leading to constitutive expression of the operon.arrow_forward
- The promoter of an operon is the site to which RNA polymerase binds to begin transcription. Certain base changes in the promoter result in a mutant site that RNA polymerase can’t bind. Would you expect mutations in the promoter that prevents binding of RNA to act in trans on another copy of the operon on a plasmid or only in cis on the copy immediately adjacent to the mutated site?arrow_forwardE. coli is given lactose as a nutrient source. This results in the activation of the lac operon only in the presence of glucose deactivation of the lac operon only in the absence of glucose deactivation of the lac operon only in the presence of glucose activation of the lac operon only in the absence of glucose Question 25 Transduction occurs when a cell takes up DNA from the environment cells are stressed a virus transfers DNA to a cell an F+ cell transfers one strand of DNA to an F- cell MacBearrow_forwardWhen lactose is present lactose induces the activation of the genes in the lac operon by binding the repressor lactose represses the activation of the genes in the lac operon by binding the repressor lactose induces the activation of the genes in the lac operon by binding the operator lactose represses the activation of the genes in the lac operon by binding the operatorarrow_forward
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