The glucose in cell culture media diffuses to the cell surface where it is rapidly consumed. The glucose concentration 3.15 g/L in the media, and the cell has a radius of 30 um. You can assume that the diffusivity of glucose in pure water (6.7 x 10^-6 cm?/s) is reasonably close to that of in media. 1. Determine the mass transfer coefficient for this situation. 2. Determine the flux of glucose at the cell surface. 3. Determine the concentration of glucose 5um from the cell surface. 4. Determine the distance from the cell at which the impact of glucose diffusion is no longer observed.
The glucose in cell culture media diffuses to the cell surface where it is rapidly consumed. The glucose concentration 3.15 g/L in the media, and the cell has a radius of 30 um. You can assume that the diffusivity of glucose in pure water (6.7 x 10^-6 cm?/s) is reasonably close to that of in media. 1. Determine the mass transfer coefficient for this situation. 2. Determine the flux of glucose at the cell surface. 3. Determine the concentration of glucose 5um from the cell surface. 4. Determine the distance from the cell at which the impact of glucose diffusion is no longer observed.
Introduction to Chemical Engineering Thermodynamics
8th Edition
ISBN:9781259696527
Author:J.M. Smith Termodinamica en ingenieria quimica, Hendrick C Van Ness, Michael Abbott, Mark Swihart
Publisher:J.M. Smith Termodinamica en ingenieria quimica, Hendrick C Van Ness, Michael Abbott, Mark Swihart
Chapter1: Introduction
Section: Chapter Questions
Problem 1.1P
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Question
The glucose in cell culture media diffuses to the cell surface where it is
rapidly consumed. The glucose concentration 3.15 g/L in the media,
and the cell has a radius of 30 um. You can assume that the diffusivity
of glucose in pure water (6.7 x 10^-6 cm?/s) is reasonably close to that of
in media.
1. Determine the mass transfer coefficient for this situation.
2. Determine the flux of glucose at the cell surface.
3. Determine the concentration of glucose 5um from the cell surface.
4. Determine the distance from the cell at which the impact of glucose
diffusion is no longer observed.
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