The following table shows the results of the chromatographic analysis of the 4 components separated isocratically using an inverted phase column with a length of 10 cm, a mobile phase flow rate of 0.2 cm/s, and a UV-Vis detector. Non retained Analite A Analite B species 0,1 PARAMETER Analite C Analite D www PEAK HEIGHT (au) 2,3 0,22 0,52 1,16 ELUSION TIME (s) 53 67 102 147 217 WIDE PEAK BASE 7 10 15 22 33 (s) a. Assuming the same fourth molar absorptivity of analytes, which analyte has the highest concentration? b. What is the selectivity factor between analytes B and C? c. Which component has the best efficiency? d. What is the theoretical plate height on the column used? e. What is the resolution between analytes A and B? does it qualify for quantitative analysis?

The following table shows the results of the chromatographic analysis of the 4 components separated isocratically using an inverted phase column with a length of 10 cm, a mobile phase flow rate of 0.2 cm/s, and a UV-Vis detector. Non retained Analite A Analite B species 0,1 PARAMETER Analite C Analite D www PEAK HEIGHT (au) 2,3 0,22 0,52 1,16 ELUSION TIME (s) 53 67 102 147 217 WIDE PEAK BASE 7 10 15 22 33 (s) a. Assuming the same fourth molar absorptivity of analytes, which analyte has the highest concentration? b. What is the selectivity factor between analytes B and C? c. Which component has the best efficiency? d. What is the theoretical plate height on the column used? e. What is the resolution between analytes A and B? does it qualify for quantitative analysis?

Chemistry

10th Edition

ISBN:9781305957404

Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Chapter1: Chemical Foundations

Section: Chapter Questions

Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...

See similar textbooks

Similar questions

A chromatographic column was found to have the following constants: A= 0.01 cm, B= 0.30 cmis and C=0.015s. What are the optimum flow rate? A none of the choices Bp-15 cmis p-45 cm/s D u-3.6 cmis E-26 cm/s
Abs at wavelength 1 (A = Abs at wavelength 2 (^ =, Sample nm) nm) Cr* Ni2+ Unknown Set up the equation for the two wavelengths using Beer's Law and calculate the concentrations of the metal ions in your unknown using two simultaneous equations: at wavelength 1: A1 = Ailccr + AılCNi at wavelength 2: A2 = A2lCcr + A2lCNi
A mixture of species of A, B, and C is injected into a liquid chromatographic column with length of 20.0 cm. The obtained separation results are listed as follows: Please calculate the resolution for species B and C from the data. Please keep your result to two decimal places. The answer would be 1.22, I would need help how to get that answer
which of the folowing(s) is/are true ? 1 split ratio in GC is a ratio of sample passed to column to amount of mobile phashe 2 column resolution decreases with increasing column lenght 3 decreasing the tube diameter ,increases the resolution
The substances in the table below were chromatographed on a gel filtration column. Estimate the molecular weight of the unknown. Compound Blue Dextran 2000 Molecular weight 2 x 106 158 000 V. (mL) 17.7 Aldolase 35.6 Catalase 32.3 210 000 Ferritin 28.6 440 000 Thyroglobulin 25.1 669 000 Unknown 30.3
Provide a structure of the fragment in the mass spec producing the peak at 139 m/z and a structure for the fragment that was lost.
Consider the following chromatographic data for the separation of methylparaben from ethylparaben in a sunscreen sample. Compound Methylparaben Retention time, tr (min). Width at base of peak, Wb (min) 3.2 4.2 0.55 0.60 Ethylparaben A compound not retained by the column in this analysis had a tm of 0.75 minutes. The column used was a C18 column with a mobile phase mixture of 75% methanol and 25% water. Calculate the number of theoretical plates for ethylparaben.
The figure below shows a portion of a GC chromatogram for a mixture of two aromatic compounds labeled A and B. The separation employed a 2.50 meter packed column under isothermal conditions (90 °C) and a flow rate of 10 mL/min. in (c) Detector Signal 300.0 250.0 200.0 150.0 100.0 50.0 0.0 1.0 Unretained 1.2 1.3 1.4 1.5 time (min) Calculate the retention factors for each peak. 1.5
Please answer this
Ab 23.