The drug pentobarbital is a sedative. It was extracted from a urine sample during a particular ultraviolet-visible spectrophotometric forensic analysis procedure. The extraction solvent was evaporated and the residue reconstituted in a small volume of the transparent solvent methanol. The pentobarbital solution thus obtained had a percent transmittance (%T) of 91 at the relevant wavelength of maximum absorbance. If the urine analyte concentration were halved, what would its percent transmittance be?
Electronic Transitions and Spectroscopy
The term “electronic” connotes electron, and the term “transition” implies transformation. In a molecule, the electrons move from a lower to a higher energy state due to excitation. The two energy states, the ground state and the excited state are the lowest and the highest energy states, respectively. An energy change is observed with this transition, which depicts the various data related to the molecule.
Photoelectron Spectroscopy
Photoelectron spectroscopy (PES) is a part of experimental chemistry. It is a technique used in laboratories that involves projecting intense beams of radiation on a sample element. In response, the element ejects electrons for which the relative energies are measured.
The drug pentobarbital is a sedative. It was extracted from a urine sample during a particular ultraviolet-visible spectrophotometric
(You may assume that the Beer-Lambert law holds).
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