Radiation of wavelength 280 nm passed through 1.0 mm of a solution that contained an aqueous solution of the amino acid tryptophan at a concentration of 0.50 mmol dm-3 . The intensity of the rad iation is reduced to 54 per cent of its init ial va lue (so T = 0.54). Calcu late the molar absorpt ion coefficient and the absorbance of tryptophan at 280 nm. What would be the transmittance through a cell of thickness 2.0 mm?
Radiation of wavelength 280 nm passed through 1.0 mm of a solution that contained an aqueous solution of the amino acid tryptophan at a concentration of 0.50 mmol dm-3 . The intensity of the rad iation is reduced to 54 per cent of its init ial va lue (so T = 0.54). Calcu late the molar absorpt ion coefficient and the absorbance of tryptophan at 280 nm. What would be the transmittance through a cell of thickness 2.0 mm?
Principles of Instrumental Analysis
7th Edition
ISBN:9781305577213
Author:Douglas A. Skoog, F. James Holler, Stanley R. Crouch
Publisher:Douglas A. Skoog, F. James Holler, Stanley R. Crouch
Chapter1: Introduction
Section: Chapter Questions
Problem 1.9QAP
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Radiation of wavelength 280 nm passed through 1.0 mm of a solution that contained an aqueous solution of the amino acid tryptophan at a concentration of 0.50 mmol dm-3 . The intensity of the rad iation is reduced to 54 per cent of its init ial va lue (so T = 0.54). Calcu late the molar absorpt ion coefficient and the absorbance of tryptophan at 280 nm. What would be the transmittance through a cell of thickness 2.0 mm?
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