The arrow in the diagram below indicates the direction of transcription. TTT AUGCCGCAAUC B TTT ATGCCGCA TACGG B G -- GTTCACG Q Search Which letter indicates the 5' end of the DNA template strand? CACTCATET LO Direction of synthesis UCACG TGC 99+ D 8368 E F 0

What Art the Features of the Series of -omes? Define the following terms: a. Genome b. Transcriptome c. Proteome d. Metabolome e. Fluxome

The Events in Transcription Initiation Describe the sequence of events involved in the initiation of transcription by E. coil RNA polymerase. Include in your description those features a gene must have for proper recognition and transcription by RNA poIymerase.

During proofreading, which of the following enzymes reads the DNA? primase topoisomerase DNA pol helicase

A frameshift mutation is caused by a: a. nucleotide substitution b. three-base insertion c. premature stop codon d. one-base insertion e. two-base deletion

About 95% of the average human transcription unit consists of: a. short repeat sequences. b. protein-coding sequences. c. regulatory sequences. d. introns. e. origins of replication.

A scientist mutates elF-2 to eliminate its GTP hydrolysis capability. How would this mutated form of elF-2 alter translation? Initiation factors would not be able to bind to mRNA The large ribosomal subunit would not be able to interact with itiRNA transcripts tRNAi-Met would not scan mRNA transcripts for the start codon elF-2 would not be able to interact with the small ribosomal subunit.

Comparison of Prokaryotic and Eukaryotic Transcription Make a list of the ways that transcription in eukaryotes differs from transcription in prokaryotes.

A normal mRNA that reads 5’ - UGCCAUGGUAAUAACACAUGAGGCCUGAAC- 3’ has an insertion mutation that changes the sequence to 5' -UGCCAUGGUUAAUAACACAUGAGGCCUGAAC- 3’. Translate the original mRNA and the mutated mRNA, and explain how insertion mutations can have dramatic effects on proteins. (Hint: Be sure to find the initiation site.)

Which of the following statements is false? a. GTP is an energy source during various stages of translation. b. In the ribosome, peptidyl transferase catalyzes peptide bondformation between amino acids. c. When the mRNA code UAA reaches the ribosome, there isno tRNA to bind to it. d. A long polypeptide is cut off the tRNA in the A site so its Metamino acid links to the amino acid in the P site. e. Forty-two amino acids of a protein are encoded by 126nucleotides of the mRNA.