Table 1: Standard Curve for Bradford Method. Absorbance measured at 595 nm Concentration of BSA Stock solution is 200 µg/mL Amount of protein in tube (ug) O ug Volume of Volume of BSA Absorbance Bradford reagent 3000 µl Cuvette no. Standard 0.00 O µl 30 µl 60 µl B 3000 µl 3000 µl 3000 µl 0.1435 1 0.2968 2 0.402 90 μι 120 µl 150 µl 0.5038 3000 µl 4 0.587 3000 µl LO

Absorbances of a series of phosphate standard solutions measured using UV- Vis spectrophotometer using 1.0 cm pathlength glass cuvette at 415 nm are given in table 2 below. 7. Table 2 Standard solution Concentration (mmol/L) Absorbance Std 1 0.0 0.1 Std 2 0.15 Std 3 0.2 0.28 Std 4 0.3 0.4 Std 5 0.4 0.55 Std 6 0.5 0.7 If an unknown sample exhibits an absorbance of 0.45. Determine the phosphate concentration in this unknown sample solution, using: a) Calibration plot b) Least-square method

Based on the data, calculate the concentration of analyte in the ORIGINAL sample in ppm. Report to 1 decimal place Sample: A 10.00 mL aliquot of the unknown was placed in a 100.0 mL volumetric flask and made up to the mark will distilled water. The absorbance of this solution is 0.185. Sample + Spike: A 10.00 mL aliquot of the unknown and a 13.00 mL aliquot of the 46.00 ppm stock were both placed in the same 100.0 mL volumetric flask and diluted to the mark with water. The absorbance of this solution is 0.326.   ( Please type answer note write by hend)

Can I have some help with this chart Calculate Absorbance from % Transmittance for each Standard and the Unknown Solution using A = - log T. • Calculate the concentration of each standard based on dilution using C1V1 = C2V2 .

Please explain on how to this problem. Thank you!

Calculate the concentration of unknown red dye solution solution is not =12.9,134,12.55,0.34

Calculate the concentration of unknown red dye solution

Sample # 1 2 0.2425 0.2160 0.2274 Weighed mass of ascorbic acid (g) volume of KIO3 (mL) 18.86 17.91 17.37 0.02459 0.02462 0.02432 [KIO3] (mol L-1) Mean [KIO3] (based on titration) 0.02451 mol L-1 Standard deviation in the mean [KIO3] mol L-1 Confidence limit for the [KI03] mol L-1 Relative confidence limit for the [KIO3] ppt =

Method of Standard Addition Worksheet You are provided a stock standard solution containing : 0.0500 g/250 mL acetaminophin (APAP). You need to determine how much standard stock is required to prepare the following solutions using 100 mL flasks. Flask 12 3 4 [APAP] added ppm (mg/L) t 10 20 30 40 RI mL Stock 0.1 What method of calibration do you use if you are analyzing a product and the product contains no ingredients that interfere with the instrument response for APAP in the product? on th LI What method of analysis do you use if you are analyzing a product and the product contains ingredients that interfere with the instrument response for APAP in the product? or

answer the following  3. What is the path length in centimeters when the molar absorptivity for a given absorbing species is 1.32 x 103L mol-1cm-1, the concentration is 0.000923 M, and the absorbance is 0.490? 4. Value for Kw at 0.0°C is 1.14 x 10-15. Calculate the pH for a neutral solution at this temperature.