Table 1: Standard Curve for Bradford Method. Absorbance measured at 595 nm Concentration of BSA Stock solution is 200 µg/mL Amount of protein in tube (ug) O ug Volume of Volume of BSA Absorbance Bradford reagent 3000 µl Cuvette no. Standard 0.00 O µl 30 µl 60 µl B 3000 µl 3000 µl 3000 µl 0.1435 1 0.2968 2 0.402 90 μι 120 µl 150 µl 0.5038 3000 µl 4 0.587 3000 µl LO

Table 1: Standard Curve for Bradford Method. Absorbance measured at 595 nm Concentration of BSA Stock solution is 200 µg/mL Amount of protein in tube (ug) O ug Volume of Volume of BSA Absorbance Bradford reagent 3000 µl Cuvette no. Standard 0.00 O µl 30 µl 60 µl B 3000 µl 3000 µl 3000 µl 0.1435 1 0.2968 2 0.402 90 μι 120 µl 150 µl 0.5038 3000 µl 4 0.587 3000 µl LO

Chemistry

10th Edition

ISBN:9781305957404

Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Chapter1: Chemical Foundations

Section: Chapter Questions

Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...

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Page of 5 O + 3 ZOOM Question 1: Using a well-known protein estimation technique, absorbance reading for a set of standards were obtained. You were required to plot the data. Which graph would you use? Plot the data using the graph type. Protein Absorbance concentration (mg/mL) (a.u.) 0.01 0.05 0.12 5 10 25 0.25 0.63 1.25 50 Question 2: Enzymes are biological catalysts and perform important biochemical reactions in the body. A group of scientists discovered a new enzyme – Enzyme X. To understand the working of this enzyme, they performed biochemical assays to assess the activity of the enzyme. The results for which are shown in the table below. Based on the information provided in the table, please state which graph would you use? Plot the data using the graph type. Enzyme activity (%) 35 55 pH 3 4 5 68 6 79 7 98 85 20 8 9 10
answer the following 3. What is the path length in centimeters when the molar absorptivity for a given absorbing species is 1.32 x 103L mol-1cm-1, the concentration is 0.000923 M, and the absorbance is 0.490? 4. Value for Kw at 0.0°C is 1.14 x 10-15. Calculate the pH for a neutral solution at this temperature.
5. Which term is INCORRECTLY defined? a. linear range - the range over which the response of an analytical method is proportional to analyte concentration b. dynamic range concentration range over which there is a measurable response to analyte, though the response is not linear c. blank solution - a solution containing all reagents and solvents used in the analysis to which no analyte is deliberately added d. standard solutions - solutions for which the standard deviation is known - 6. True or False: Internal standards correct random error caused by run-to-run variation. a. True b. False
A standard curve was produced using varying concentrations of KCl and the results are shown below. A sample of pond water was found to have a conductivity of 535 µS/cm. What is the concentration of total dissolved solids in the water sample? Recall the TDS constant is 0.67. CONDUCTIVITY (MICROS 1600 1400 1200 1000 800 600 200 0 Standard Curve of Conductivity vs Concentration Concentrations (M) 0.002 A. 392 mg/L B. 478 mg/L ✓C. 798 mg/L D. 247 mg/L 0.004 y = 132208x + 19.045 R²-0.9996 0.006 CONCENTRATION (M) 0.008 0.01 0.012
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Mm.30.