Table 1: Standard Curve for Bradford Method. Absorbance measured at 595 nm Concentration of BSA Stock solution is 200 µg/mL Amount of protein in tube (ug) O ug Volume of Volume of BSA Absorbance Bradford reagent 3000 µl Cuvette no. Standard 0.00 O µl 30 µl 60 µl B 3000 µl 3000 µl 3000 µl 0.1435 1 0.2968 2 0.402 90 μι 120 µl 150 µl 0.5038 3000 µl 4 0.587 3000 µl LO

Table 1: Standard Curve for Bradford Method. Absorbance measured at 595 nm Concentration of BSA Stock solution is 200 µg/mL Amount of protein in tube (ug) O ug Volume of Volume of BSA Absorbance Bradford reagent 3000 µl Cuvette no. Standard 0.00 O µl 30 µl 60 µl B 3000 µl 3000 µl 3000 µl 0.1435 1 0.2968 2 0.402 90 μι 120 µl 150 µl 0.5038 3000 µl 4 0.587 3000 µl LO

Chemistry

10th Edition

ISBN:9781305957404

Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Chapter1: Chemical Foundations

Section: Chapter Questions

Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...

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Absorbances of a series of phosphate standard solutions measured using UV- Vis spectrophotometer using 1.0 cm pathlength glass cuvette at 415 nm are given in table 2 below. 7. Table 2 Standard solution Concentration (mmol/L) Absorbance Std 1 0.0 0.1 Std 2 0.15 Std 3 0.2 0.28 Std 4 0.3 0.4 Std 5 0.4 0.55 Std 6 0.5 0.7 If an unknown sample exhibits an absorbance of 0.45. Determine the phosphate concentration in this unknown sample solution, using: a) Calibration plot b) Least-square method
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Sample # 1 2 0.2425 0.2160 0.2274 Weighed mass of ascorbic acid (g) volume of KIO3 (mL) 18.86 17.91 17.37 0.02459 0.02462 0.02432 [KIO3] (mol L-1) Mean [KIO3] (based on titration) 0.02451 mol L-1 Standard deviation in the mean [KIO3] mol L-1 Confidence limit for the [KI03] mol L-1 Relative confidence limit for the [KIO3] ppt =
Method of Standard Addition Worksheet You are provided a stock standard solution containing : 0.0500 g/250 mL acetaminophin (APAP). You need to determine how much standard stock is required to prepare the following solutions using 100 mL flasks. Flask 12 3 4 [APAP] added ppm (mg/L) t 10 20 30 40 RI mL Stock 0.1 What method of calibration do you use if you are analyzing a product and the product contains no ingredients that interfere with the instrument response for APAP in the product? on th LI What method of analysis do you use if you are analyzing a product and the product contains ingredients that interfere with the instrument response for APAP in the product? or