Suppose you specifically want to measure the amount of beta-glucose present in a sample. You have an enzyme that reacts specifically with the beta form of the sugar,and produces a signal that is easy to measure. However, every time you try to measure the amount of beta-glucose, mutarotation occurs and the alpha form of the sugar is converted into beta!! Can you suggest a simple way of overcoming this problem so you could measure only the beta form of the sugar? HINT: Is there a way you can prevent mutarotation?
Nucleotides
It is an organic molecule made up of three basic components- a nitrogenous base, phosphate,and pentose sugar. The nucleotides are important for metabolic reactions andthe formation of DNA (deoxyribonucleic acid) and RNA (ribonucleic acid).
Nucleic Acids
Nucleic acids are essential biomolecules present in prokaryotic and eukaryotic cells and viruses. They carry the genetic information for the synthesis of proteins and cellular replication. The nucleic acids are of two types: deoxyribonucleic acid (DNA) and ribonucleic acid (RNA). The structure of all proteins and ultimately every biomolecule and cellular component is a product of information encoded in the sequence of nucleic acids. Parts of a DNA molecule containing the information needed to synthesize a protein or an RNA are genes. Nucleic acids can store and transmit genetic information from one generation to the next, fundamental to any life form.
Please asnwer the following question:
Suppose you specifically want to measure the amount of beta-glucose present in a sample.
You have an enzyme that reacts specifically with the beta form of the sugar,and produces a signal that is easy to measure.
However, every time you try to measure the amount of beta-glucose, mutarotation occurs and the alpha form of the sugar is converted into beta!!
Can you suggest a simple way of overcoming this problem so you could measure only the beta form of the sugar?
HINT: Is there a way you can prevent mutarotation?
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