Suppose an organelle contains 0.03 M KCI and 25 mM NaCl. How many grams of sucrose would you need per liter of solution to isolate intact organelles at 25 degrees Celsius (room temperature)? R is the gas constant = 8.315 J/molk T is the absolute temperature in Kelvins. Answer with 2 decimal places as before.

A solution containing 3.58 x 1023 molecules/m3 of protein in water is separated from pure water by a membrane 3.20 μm thick.  The diffusion coefficient of the protein through the membrane is 7.15 x 10-18 m2/s.  On average, how many molecules cross 0.0240 μm2 of this membrane each second? [Answer as a positive number with 3 sig digits, but do not enter units with your answer]

Give typing answer with explanation and conclusion Correct answer ?

A beaker contains two compartments (A and B) with equal volumes of solution separated by an artificial membrane with a pore size of 24 Angstrom (Å). Explain the net movement of solute if Compartment A has 3% albumin while compartment B has 2% potato starch (albumin diameter = 38 Å; potato starch = 300,000-1000000 Å).

The average time it takes for a molecule to diffuse adistance of x cm is given byt = x2/2D where t is the time in seconds and D is the diffusioncoefficient. Given that the diffusion coefficient ofglucose is 5.7 × 10−7cm2/s, calculate the time it wouldtake for a glucose molecule to diffuse 10 μm, which isroughly the size of a cell.

The diameter of a mitochondrion is about 1.2 ✕ 10−4 in. What is its diameter in centimeters?

In an experiment, a 0.001 (mole fraction) solution of polysaccharide in water is made and is placed in the compartment A (see Figure below).  Compartment B is filled with pure water.  The two compartments are separated by a porous semi-permeable membrane that allows the exchange of water molecules between the two compartments, but not that of the larger polysaccharide molecules                             a) Show that the chemical potential of water in compartment A is lower than that in compartment B by 2.48 J/mol. b) As a result of this chemical potential difference, water molecules will move from compartment B to compartment A. This causes the pressure in compartment A, relative to that in B, to increase. How would this affect the chemical potential of water in compartment B? When would the diffusion of water from B to A cease (i.e. equilibrium is achieved)? c) Using your answer to part (b), work out the difference between the pressure in compartment A and B when…

The simple form of |Hoff equation is: II = [B]RT In this equation the [B] is the molar concentration of solute. So: n m [B] = v MV = cg /MA Where c, the mass concentration of the solute is in the total volume of solution and M, is the molar mass of the solute. This equation can be replaced in the previous one to get: RT II = MA In this equation molar mass of given solute can be detemined from the slope of the II vs Cz plot. This equation applies only to solutions that are sufficiently dilute to behave as ideal-dilute solutions. In the case of non-ideal solutions, however, the extended formula is: II = [B]RT{1+ k. [B] + n. [B]² + ...} Biological macromolecules dissolve to produce solutions that are far from ideal, but we can still calculate the osmotic pressure by assuming that the van't Hoff equation is only the first term of a lengthier expression: II [B]RT(1+ b. [B]) II = RT + bRT. [B] [B] II = RT + bRT./M. */Ma п RT ÞRT Ca MA MA In this equation molar mass of given biomolecule can…

Ammonia (NH3) is a weak base that under acidic conditions becomes protonated to the ammonium ion in the  following reaction: NH3   +  H+   →   NH+4 NH3 freely permeates biological membranes, including those of lysosomes. The lysosome is a subcellular organelle with a pH of about 4.5–5.0; the pH of cytoplasm is about 7.0. What is the effect on the pH of the fluid content of lyso- somes when cells are exposed to ammonia? Note: Ammo- nium (NH4+) does not diffuse freely across membranes.

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