Ammonia (NH3) is a weak base that under acidic conditions becomes protonated to the ammonium ion in the  following reaction: NH3   +  H+   →   NH+4 NH3 freely permeates biological membranes, including those of lysosomes. The lysosome is a subcellular organelle with a pH of about 4.5–5.0; the pH of cytoplasm is about 7.0. What is the effect on the pH of the fluid content of lyso- somes when cells are exposed to ammonia? Note: Ammo- nium (NH4+) does not diffuse freely across membranes.

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A:

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Osmosis

In simple words, osmosis is the slow, gradual movement of a liquid through a membrane. But there are nuances to this process. For instance, there has to be an imbalance in the distribution of solute in the liquid on either side of the membrane, which has to be semipermeable. The liquid in the region with a lower solute concentration moves to the region with a higher concentration, thereby displacing the solution in the process. The objective is to equalize the concentration of molecules on both sides of the membrane; the displacement of liquid can be viewed as a byproduct of this equalization. Since there is no energy required to carry osmosis, it is considered a passive process.