Supply the Wuras in the blanks below: In an inducible operon, transcription is normally and must be turned In a repressible operon, transcription is normally. and must be turned Briefly describe the overall function of the structural genes in the lac operon.

Briefly describe the lac operon and how it controls the metabolism of lactose.

For each of the following conditions in the Lac Operon, state whether transcription will occur or not and state why. Include all the major actors including glucose, lactose, cAMP and CAP(CPR). Key + (present) - (absent) 1) Glucose-; Lactose- 2) Glucose+; Lactose- 3) Glucose+; Lactose+ 4) Glucose-; Lactose+

Which of the following is true about the Lac operon? Group of answer choices An operon with a constitutive operator (O^C mutation) will transcribe in the presence of a super repressor It is a positive repressible operon that is influenced mainly by fluctuations in glucose levels Produces a large quantity of tryptophan in order to synthesize lactase It is found in eukaryotes and is responsible for creating an enzyme that breaks down lactose

The tryptophan operon of E. coli represents an example of a gene regulatory system which is: a repressible system (normally turned on, but can be repressed) a repressible system (normally repressed, but can be turned on) an activator system (normally turned off, but can be activated) an activator system (normally activated, but can be turned off) all of the above

Explain and give an example (lac operon) of how inducer operons function in prokaryotes.

Given the Table below concerning the lac operon, match the letter with what would be occurring for each scenario. Repressor is bound to the operator, cAMP complex is bound and transcription is prevented.   Allolactose is bound to the repressor, cAMP complex is bound, and transcription is prevalent.                                    Allolactose is bound to the repressor, cAMP complex is not assembled, and transcription is slow                        Repressor is bound to the operator, cAMP complex is not assembled, and transcription is prevented.

What is a specific region in the trp operon that you could mutate so it expresses the trp operon in conditions of excess tryptophan. Why will this mutation cause this? Operon structure: promoter, operater, structural genes promoter -  DNA region that is the site of RNA polymerase sigma binding and transcription initiation operator – DNA region that influences gene expression through its interaction with a protein (transcription factor) structural genes – gene whose expression is being regulated; encodes a polypeptide or functional RNA

Describe in details all the differences between The Trp Operon and the Lac Operon. Why one is always off while the other is always ON? Can you find examples for other catabolic and anabolic pathways that could behave in the same way?

The organization and function of the lac operon in E. coli is shown in the following figures: XXI Transcription Repressor mRNA Translation RNA polymerase M M Flask ZYA XXXX Active M-repressor protein Repressor active, operon off. The repressor protein binds with the operator, preventing transcription from the operon. (a) An inducible operon Copyright © 2007 Pearson Education, Inc., publishing as Benjamin Cummings Allolactose (inducer) Carbon/Energy Source 0 Z MInactive repressor protein (a) An inducible operon Transcription Translation XXX Permease B-Galactosidase Transacetylase Operon mRNA 3 Repressor inactive, operon on. When the inducer allolactose binds to the repressor protein, the inactivated repressor can no longer block transcription. The structural genes are transcribed, ultimately resulting in the production of the enzymes needed for lactose catabolism. Copyright © 2007 Pearson Education, Inc., publishing as Benjamin Cummings Suppose you inoculate three flasks of minimal…