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The purpose of the progress curve is to determine if an enzymatic reaction rate remains constant for given concentrations of enzyme and substrate, for a given assay time (20 min). How long did the rate of reaction of WGAP remain constant in your experiments? How do you know? What would cause the reaction rate to not be constant and plateau after some time?

The purpose of the progress curve is to determine if an enzymatic reaction rate remains constant for given concentrations of enzyme and substrate, for a given assay time (20 min). How long did the rate of reaction of WGAP remain constant in your experiments? How do you know? What would cause the reaction rate to not be constant and plateau after some time?

Biochemistry
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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The purpose of the progress curve is to determine if an enzymatic reaction rate remains constant for given concentrations of enzyme and substrate, for a given assay time (20 min). How long did the rate of reaction of WGAP remain constant in your experiments? How do you know? What would cause the reaction rate to not be constant and plateau after some time?
27 28 Part B: progr Abs 29 30 31 32 33 34 35 36 37 38 39 40 41 42 44 5 5 10 10 15 15 20 20 25 25 Unmol p-np 0.109 0.012868 0.133 0.015701 0.294 0.034708 0.29 0.034236 0.386 0.045569 0.329 0.03884 0.402 0.047458 0.487 0.057493 0.511 0.060326 0.596 0.070361 "Mur amount of p-np (umoles) 0.08 0.07 0.06 0.05 0.04 0.03 0.02 0.01 0 0 5 10 15 Reaction time (min) y=0.0027x 20 25 30
Transcribed Image Text:27 28 Part B: progr Abs 29 30 31 32 33 34 35 36 37 38 39 40 41 42 44 5 5 10 10 15 15 20 20 25 25 Unmol p-np 0.109 0.012868 0.133 0.015701 0.294 0.034708 0.29 0.034236 0.386 0.045569 0.329 0.03884 0.402 0.047458 0.487 0.057493 0.511 0.060326 0.596 0.070361 "Mur amount of p-np (umoles) 0.08 0.07 0.06 0.05 0.04 0.03 0.02 0.01 0 0 5 10 15 Reaction time (min) y=0.0027x 20 25 30
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