Biochemistry
Biochemistry
9th Edition
ISBN: 9781319114671
Author: Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher: W. H. Freeman
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Question

ANSWER E PLS

(e) What kind of inhibitor is it likely to be? Plot the data in Lineweaver-Burk plot form to support your answer.
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Transcribed Image Text:(e) What kind of inhibitor is it likely to be? Plot the data in Lineweaver-Burk plot form to support your answer.
You are in the laboratory and measure the initial rate of an enzyme reaction as a function of substrate concentration in the presence and absence of an inhibitor. You obtain the following data: [Substrate], mM Vo, - inhibitor, (μM/min) Vo, + inhibitor, (μM/min) 0.0001 33 17 0.0002 50 29 0.0005 71 50 0.001 83 67 0.002 91 80 0.005 96 91 0.01 98 95 0.02 99 98 0.05 100 99 0.1 100 100 0.1 100 100
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Transcribed Image Text:You are in the laboratory and measure the initial rate of an enzyme reaction as a function of substrate concentration in the presence and absence of an inhibitor. You obtain the following data: [Substrate], mM Vo, - inhibitor, (μM/min) Vo, + inhibitor, (μM/min) 0.0001 33 17 0.0002 50 29 0.0005 71 50 0.001 83 67 0.002 91 80 0.005 96 91 0.01 98 95 0.02 99 98 0.05 100 99 0.1 100 100 0.1 100 100
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Step 1: Michaelis Menten equation and Lineweaver Burk Plot
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Step 2: Lineweaver Burk plot for the given enzyme
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Step 3: Interpretation of the LB Plot
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Step 4: Competitive inhibitors
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Solution
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