Steroid hormones are often present in cells in low concentrations that make them difficult toisolate and identify. Researchers find it easier to isolate the proteins that bind steroids usingaffinity chromatography. Explain how you would use this technique to isolate a proteinsuspected of steroid hormone binding

Q: Mucolipidosis type X is a lysosomal storage disease that was suggested to be a result of impaired…

A: Lysosomal storage disease is mainly detected by a blood test using a substrate called 4-…

Q: Proteinase K should be used as the first step of DNA purification (before applying phenol CIA…

A: Proteins are large, complex molecules made up of chains of amino acids that perform various…

Q: prpc is a normal protein of our neuronal cell membrane that is sensitive to protease action (ie can…

A: The proteases are the protein degrading enzymes. Once the role of proteinaceous enzymes and…

Q: Human serum transferrin is an iron transport protein. With the use of appropriate diagrams, describe…

A: Transferrin protein is a protein complex, which is known to transfer iron molecules to the cell.

Q: Glucose 6-Pase is an enzyme present in liver cells but not in musc cells. The enzyme is bound to the…

A: Enzymes are protein molecules that increase the rate of reaction by decreasing the activation…

Q: What structural features make glycans good cell-surface markers? What are the main challenges to…

A: Step 1 Glycans are sugar molecules that are often attached to proteins and lipids on the surface of…

Q: What are the differences between synthesis of phosphatidylethanolamine in prokaryotes and…

A: Glycerol is a polyol compound that is made up of more than one hydroxyl group. Glycerol is a…

Q: proteins are deamidated in an aqueous solution to produce ammonium and aspartate, respectively, from…

A: Isoelectric focusing (IEF), also known as electrofocusing, is a technique for separating different…

Q: A mixture of lipids containing phosphatidic acid, cholesterol, testosterone, phosphatidylserine, and…

A: Non-polar molecules do not form a hydrogen bond with water can are thus considered hydrophobic.…

Q: a protein collected through affinity chromatography displays no activity even though it is found to…

A: Protein is made up of one or more amino acids that are linked together to form a chain. The building…

Q: Polyphosphate (poly-P) Exopolysaccharides (EPS) • Autoinducers (AI) • Guanidine tetraphosphate…

A: Answers Rnase is an enzyme that is used to degrade the RNA molecules. While HipAB is a…

Q: Which of these situations would produce a Hill plot with a Hill coefficient less than 1? A purified…

A: A Hill plot is used to establish the cooperativity of ligand binding to a protein.NH (Hill…

Q: In a mixture of five proteins listed, draw an elution profile (Absorbance vs. mL eluted,  arbitrary)…

A: Size-exclusion chromatography also known as gel filtration chromatography, is a chromatographic…

Q: Saccharide M, a sugar made of repeating units of maltose and cellobiose, is a component of…

A: Glycoproteins are consisted of protein and glycan moiety which is involved in several biological…

Q: Polychlorinated biphenyls (PBCS) are a group of chemicals with a wide range of uses in manufacturing…

A: Polychlorinated Biphenyls (PBCs) are a group of chemicals with a wide range of uses in…

Q: Why is COVID-19 a man made? (Brief explanation only atleast 3-5 sentences)

A: Coronavirus is  large family of virus that are  the source of respiratory infection, including the…

Q: Compounds A, B, C, and D are known to be intermediates in the pathway for production of protein E.…

A: In the question, E protein production pathway involves intermediates A, B, C, and D. Mutation or…

Q: You want to make 94 µL of the diluted Cell-Free extract for estimation of total protein. To do this,…

A: A ratio of 1:25 means add 1 part of product to 25 parts of water. Dilution factor does not gives…

Q: Please pick among these 4 Protein chromatography is typically column-based: — lon-exchange —…

A: Column chromatography is one of the most common methods of protein purification based on the…

Q: a) From the chemical structure above, write the amino acid sequence using both three-letter and…

A: Amino acids are basic subunit of peptides (2 to 50 amino acids), peptides is formed by the linking…

Q: the key features between glycosaminoglycan-binding proteins and lectins?

A: Glycosaminoglycans (GAGs) are mucopolysaccharides containing a large number of disaccharide units…

Q: One can identify phenylketonurics and PKU carriers (heterozygotes) by means of a phenylalanine…

A: The graph that shows different patients at different conditions between relative serum…

Q: A polyhistidine-tag or better known by its trademarked name His-tag is an amino acid motif present…

A: In vectors used to produce recombinant proteins, the DNA sequence defining a string of six to nine…

Q: a. What is the optimum pH of wild type β-galactosidase?         _________ b. What is the optimum…

A: An enzyme is  shows its maximal activity at its optimal condition like pH, temperature, substrate.

Q: Certain glutamine analogs irreversibly inactivate enzymes that bind glutamine. Identify the…

A: Nucleotides are ubiquitous as they are involved in majority of biochemical reactions. All of the…

Q: Which of the following identifies the dependent variable in this experiment? A the concentration of…

A: INTRODUCTION : During any exercise or physical workout, lactate will be a byproduct formed when…

Q: High salt concentrations tend to cause protein aggregation. Suggest a way to identify proteins…

A: Salting out is a process based on protein solubility. The globular proteins are precipitate in the…

Q: Protein kinases (PTKS) are enzymes that regulate the biological activity of proteins by…

A: CELL CYCLE ❅ The process by which a parent cell divides into two daughter cells. ❅ The cell cycle…

Q: ( blood lactate (millimale/liter) start A Exercise с - oxygen supplemented mater blood lactate…

A: Lactate accumulation has been found to be minimised through supplementation of oxygenated water as…

Q: calculate the molecular weights of proteins

A: Amino acids are the monomer units of peptides and proteins and they vary from 89 Dalton (Alanine) to…

Q: 1) Draw a chromatogram depicting separation of proteins 1, 2, and 3 (protein #1, mw 30,000, protein…

A: The diagram of chromatograph  is as follows-

Q: What are eicosanoids? Mention their biomedical importance.

A: Introduction: The below answer provides an explanation of eicosanoids, their functions, and their…

Q: A protein has a molecular mass of 400 kDa when measured by size-exclusion chromatography. When…

A: Size exclusion chromatography is a technique which separates molecules according to their size…

Q: Monoclonal antibodies can be conjugated to an insoluble support by chemical methods. Explain how…

A: The cloning of immune cells results in the generation of similar immunoglobulins called monoclonal…

Q: Hormone A binds to its receptor with a KL of 5 μM. In the presence of 2.5 μM compound B and 2.0 μM…

A: A ligand is a substance that forms a complex with a biomolecule to serve a biological purpose. In…

Q: Given that the protein concentration is 0.25 μM, what is the association constant (KA) of the…

A: Fluorescence is a luminescence technique used in fluorescent labeling and fluorescence spectroscopy…

Q: Suppose that you are tasked to determine the protein concentration of an unknown protein solution…

A: The concentration of unknown protein solution can be determined by plotting a graph between…

Q: Amoeboid cells that migrate through our tissues, such as the class of white blood cells known as…

A: RAC is responsible for protrusion as well as attachment and thus, dominates at the leading edge…

Metabolic pathways

Metabolism is defined as the group of life-supporting, enzyme-mediated chemical reactions in organisms. A metabolic pathway is a set of these chemical reactions that involve anabolism (synthesis of complex biomolecules) and catabolism (breakdown of biomolecules into small molecules) that occur inside a cell. Metabolites are the reacting substances, products, and intermediate substances of an enzymatic process that are altered by a series of chemical events catalyzed by enzymes.